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MB Sample ID: SA096040
| Local Sample ID: | HH_7 |
| Subject ID: | SU001398 |
| Subject Type: | Invertebrate |
| Subject Species: | Haliotis discus hannai |
| Taxonomy ID: | 42344 |
| Weight Or Weight Range: | 2.17 ± 0.56 g |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001398 |
| Subject Type: | Invertebrate |
| Subject Species: | Haliotis discus hannai |
| Taxonomy ID: | 42344 |
| Weight Or Weight Range: | 2.17 ± 0.56 g |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| HH_7 | SA096040 | FL013575 | - | Acclimatization temperature |
| HH_7 | SA096040 | FL013575 | group H exposure to 31°C for 3hours | Heat exposure |
Collection:
| Collection ID: | CO001393 |
| Collection Summary: | Hepatopancreas tissue was dissected from four groups of juvenile abalones (L, H, LH and HH) on ice, washed by deionized water and drain with a neutral filter. |
| Sample Type: | new |
Treatment:
| Treatment ID: | TR001413 |
| Treatment Summary: | Within each group, tissues from three abalones were mixed together and ground into powder in liquid nitrogen for subsequent metabolite extraction and metabonomics-based analysis. In this way, nine biological replications were produced for each group. Samples were stored in liquid nitrogen. |
Sample Preparation:
| Sampleprep ID: | SP001406 |
| Sampleprep Summary: | 50 mg of sample was taken and placed in an Eppendorf tube, followed by the addition of 1000 µl extraction solvent containing an internal target (V methanol : V acetonitrile : V water = 2 : 2 : 1, containing internal standard 2 µg/ml). The mixture was homogenized in ball mill for 4 min at 45 Hz, then ultrasound treated for 5min (incubated in ice water). After homogenization for 3 times, the mixture was incubated for 1 h at -20 °C to precipitate proteins. Centrifugation was performed at 12000 rpm for 15 min at 4 °C. A total of 825 µl supernatant was carefully transferred into a fresh Eppendorf tube, and dried in a vacuum concentrator without heating. 200 µl extraction solvent (V acetonitrile: V water = 1:1) was then added for reconstitution, followed by vortex 30 s and sonicated for 10 min (4 °C water bath), centrifuge for 15 min at 12000 rpm (4 °C). 75 µl supernatant was transferred into a fresh 2 ml LC/MS glass vial for UHPLC-QTOF-MS analysis, while another 20 µl was taken from each sample and pooled as QC samples. Six technical replicates were conducted to QC sample with 75 µL each for UHPLC-QTOF-MS analysis. |
| Sampleprep Protocol Filename: | LC-MS-Q211.pdf |
Combined analysis:
| Analysis ID | AN002202 | AN002203 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1290 | Agilent 1290 |
| Column | Waters Acquity UPLC BEH amide,(100 x 2.1mm,1.7um,100 Å) | Waters Acquity UPLC BEH amide,(100 x 2.1mm,1.7um,100 Å) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | ABI Sciex 5600 TripleTOF | ABI Sciex 5600 TripleTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | mV*s | mV*min |
Chromatography:
| Chromatography ID: | CH001616 |
| Chromatography Summary: | UHPLC |
| Methods Filename: | LC-MS-Q211.pdf |
| Instrument Name: | Agilent 1290 |
| Column Name: | Waters Acquity UPLC BEH amide,(100 x 2.1mm,1.7um,100 Å) |
| Flow Gradient: | 40%-95% B |
| Flow Rate: | 500 µL/min |
| Solvent A: | 100% water; 25 mM ammonium acetate, pH 9.75 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS002048 |
| Analysis ID: | AN002202 |
| Instrument Name: | ABI Sciex 5600 TripleTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | MS raw data files were converted to the mzXML format using ProteoWizard, and processed by R package XCMS (version 3.2). The preprocessing results generated a data matrix that consisted of the retention time, mass-to-charge ratio (m/z) values, and peak intensity. R package CAMERA was used for peak annotation after XCMS data processing. |
| Ion Mode: | POSITIVE |
| MS ID: | MS002049 |
| Analysis ID: | AN002203 |
| Instrument Name: | ABI Sciex 5600 TripleTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | MS raw data files were converted to the mzXML format using ProteoWizard, and processed by R package XCMS (version 3.2). The preprocessing results generated a data matrix that consisted of the retention time, mass-to-charge ratio (m/z) values, and peak intensity. R package CAMERA was used for peak annotation after XCMS data processing. |
| Ion Mode: | NEGATIVE |
