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MB Sample ID: SA096450
| Local Sample ID: | JW44-NP |
| Subject ID: | SU001403 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | 5-6 months or 26 months |
| Animal Animal Supplier: | NIA |
| Animal Housing: | UW ARCF |
| Animal Feed: | Ad libitum |
| Animal Water: | Ad libitum |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001403 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | 5-6 months or 26 months |
| Animal Animal Supplier: | NIA |
| Animal Housing: | UW ARCF |
| Animal Feed: | Ad libitum |
| Animal Water: | Ad libitum |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| JW44-NP | SA096450 | FL013602 | 26 | Age (Months) |
| JW44-NP | SA096450 | FL013602 | SS-31 (3 mg/kg/day) + NMN (300 mg/kg/day) | Treatment |
Collection:
| Collection ID: | CO001398 |
| Collection Summary: | Hearts were removed from mice immediately following live cervical dislocation euthanasia. Hearts were flushed with sterile normal saline, cut into large chunks, and snap frozen in liquid nitrogen. |
| Sample Type: | Heart |
Treatment:
| Treatment ID: | TR001418 |
| Treatment Summary: | Mice were treated with SS-31, NMN, neither, or both for 8 weeks. SS-31 was administered through subdermal osmotic pumps at a 3 mg/kg body weight/day dose. NMN was administered through drinking water at a 300 mg/kg body weight/day dose. |
| Treatment Compound: | SS-31 and NMN |
| Treatment Route: | Subdermal osmotic pump for SS-31, drinking water for NMN |
| Treatment Dose: | 3 mg/kg body weight/day for SS-31, 300 mg/kg body weight/day for NMN |
Sample Preparation:
| Sampleprep ID: | SP001411 |
| Sampleprep Summary: | Heart tissue samples were homogenized in water and methanol with spiked stable isotopes as internal QC standards. Samples were purified by pelleting proteins, drying of the solution via Speedvac (Thermo Fisher Scientific), and reconstituting in HILIC solvent. |
Combined analysis:
| Analysis ID | AN002215 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Shimadzu Nexera XR LC-20AD |
| Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 6500 QTrap |
| Ion Mode | UNSPECIFIED |
| Units | Peak Area |
Chromatography:
| Chromatography ID: | CH001625 |
| Chromatography Summary: | Samples were injected into a chromatography system consisting of a dual injection valve setup allowing injections onto two different LC columns with each column dedicated to an ESI polarity. 5 µL were injected on the positive mode column and 10 µL on the negative side column. The columns were a matched pair from the same production lot number and were both a Waters XBridge BEH amide column (2.1 x 150 mm). Autosampler was maintained at 4 °C and column oven was set to 40 °C. 0.3 mL/min gradient was 0-3 min 95% B, 3-8 min 95 -> 50% B, 8-12 min 50% B, 12-13 min 50 – 95% B, 13-18 min 95% B. After completion of the 18 minute gradient, injection on the opposite column was initiated and the inactive column was allowed to equilibrate at starting gradient conditions. A set of QC injections for both instrument and sample QC were run at the beginning and end of the sample run. |
| Instrument Name: | Shimadzu Nexera XR LC-20AD |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Column Temperature: | 40 |
| Solvent A: | 95% water/3% acetonitrile/2% methanol; 0.2% acetic acid; 10 mM ammonium acetate |
| Solvent B: | 93% acetonitrile/5% water/2% methanol; 0.2% acetic acid; 10 mM ammonium acetate |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS002061 |
| Analysis ID: | AN002215 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Samples were injected into a chromatography system consisting of a dual injection valve setup allowing injections onto two different LC columns with each column dedicated to an ESI polarity. 5 µL were injected on the positive mode column and 10 µL on the negative side column. The columns were a matched pair from the same production lot number and were both a Waters XBridge BEH amide column (2.1 x 150 mm). Autosampler was maintained at 4 °C and column oven was set to 40 °C. 0.3 mL/min gradient was 0-3 min 95% B, 3-8 min 95 -> 50% B, 8-12 min 50% B, 12-13 min 50 – 95% B, 13-18 min 95% B. After completion of the 18 minute gradient, injection on the opposite column was initiated and the inactive column was allowed to equilibrate at starting gradient conditions. A set of QC injections for both instrument and sample QC were run at the beginning and end of the sample run. Data was integrated by MultiQuant 3.0.2 or Sciex-OS v1.5 software. Peaks were selected based on peak shape, a signal-to-noise of 10 or better and retention times consistent with previously run standards and sample sets. |
| Ion Mode: | UNSPECIFIED |
