Return to study ST001349 main page
MB Sample ID: SA097916
| Local Sample ID: | cow 20_Day 14 |
| Subject ID: | SU001423 |
| Subject Type: | Mammal |
| Subject Species: | Bos taurus |
| Taxonomy ID: | 9913 |
| Gender: | Female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001423 |
| Subject Type: | Mammal |
| Subject Species: | Bos taurus |
| Taxonomy ID: | 9913 |
| Gender: | Female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| cow 20_Day 14 | SA097916 | FL013735 | 14 | Day |
| cow 20_Day 14 | SA097916 | FL013735 | treatment | Group |
| cow 20_Day 14 | SA097916 | FL013735 | multiparous | Parity |
Collection:
| Collection ID: | CO001418 |
| Collection Summary: | Blood samples were collected before the morning feeding from the jugular vein of all animals using vacutainer tubes. Samples were allowed to clot at 25°C for 2 h and all tubes were then centrifuged at 2000 × g at 4°C for 15 min to separate serum. Serum was pipetted into 2 mL tubes and stored at -80°C for further analysis. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR001438 |
| Treatment Summary: | The feed contained either no additive as a control (CON) or a mineral-based endotoxin binder (EB). |
Sample Preparation:
| Sampleprep ID: | SP001431 |
| Sampleprep Summary: | Determination of the serum metabolome was carried out using a targeted metabolomics approach based on the Biocrates MxP® Quant 500 kit. Ten μL aliquots of the serum samples were processed according to the manufacturer’s instructions. |
Combined analysis:
| Analysis ID | AN002243 | AN002244 | AN002245 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | None (Direct infusion) |
| Chromatography system | Agilent 1290 | Agilent 1290 | Agilent 1290 |
| Column | - | - | - |
| MS Type | ESI | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole |
| MS instrument name | Sciex 6500+ QTrap | Sciex 6500+ QTrap | Sciex 6500+ QTrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE |
| Units | µM | µM | µM |
Chromatography:
| Chromatography ID: | CH001649 |
| Chromatography Summary: | RP-LC-MS/MS |
| Instrument Name: | Agilent 1290 |
| Column Name: | - |
| Column Temperature: | 50 °C |
| Solvent A: | 100% water; 0.2% formic acid |
| Solvent B: | 100% acetonitrile; 0.2% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001650 |
| Chromatography Summary: | FIA-MS/MS |
| Instrument Name: | Agilent 1290 |
| Column Name: | - |
| Column Temperature: | 25 °C |
| Solvent A: | none |
| Solvent B: | 290 mL of methanol plus 10 mL of Biocrates' FIA solvent ampule |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS002089 |
| Analysis ID: | AN002243 |
| Instrument Name: | Sciex 6500+ QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | POSITIVE |
| MS ID: | MS002090 |
| Analysis ID: | AN002244 |
| Instrument Name: | Sciex 6500+ QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | NEGATIVE |
| MS ID: | MS002091 |
| Analysis ID: | AN002245 |
| Instrument Name: | Sciex 6500+ QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | POSITIVE |
