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MB Sample ID: SA098014
Local Sample ID: | Sample 101 |
Subject ID: | SU001424 |
Subject Type: | Yeast |
Subject Species: | Saccharomyces cerevisiae |
Taxonomy ID: | 4932 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001424 |
Subject Type: | Yeast |
Subject Species: | Saccharomyces cerevisiae |
Taxonomy ID: | 4932 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Sample 101 | SA098014 | FL013781 | 16 | Timepoint |
Sample 101 | SA098014 | FL013781 | 5 | Replicate |
Collection:
Collection ID: | CO001419 |
Collection Summary: | Samples were collected from fermentor eppendorf bioflo 120 through different timepoints of the Yeast Metabolic Cycle. Cells were snap frozen on liquid nitrogen for metabolite extraction. |
Sample Type: | Yeast cells |
Treatment:
Treatment ID: | TR001439 |
Treatment Summary: | Cells were not treated, this was a timecourse on stable conditions, that yeast cells use to enter an ultradian rythm, which was measured during the timecourse. |
Sample Preparation:
Sampleprep ID: | SP001432 |
Sampleprep Summary: | Cell pellets were lyophilized to dryness overnight. The lyophilized pellet was homogenized in 50:50 acetonitrile/water with 0.3 % formic acid for OAs and AAs, 5% TCA for acetyl and malonyl CoA, 0.5M PCA for oxidized nucleotides and 50:50 methanol/NaOH for reduced nucleotides using the Precellys bead-based homogenizing system. Precellys tough micro-organism lysing kit (VK-05) was used to burst the yeast cells. An aliquot of homogenate for the required assay was aliquoted and immediately stored at -80oC. |
Combined analysis:
Analysis ID | AN002246 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 |
Column | Hypercarb |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Thermo Quantiva |
Ion Mode | POSITIVE |
Units | nmoles/mg protein |
Chromatography:
Chromatography ID: | CH001651 |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Hypercarb |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS002092 |
Analysis ID: | AN002246 |
Instrument Name: | Thermo Quantiva |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | The raw data was processed using Xcalibur 3.0. |
Ion Mode: | POSITIVE |