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MB Sample ID: SA098034
| Local Sample ID: | Sample 86 |
| Subject ID: | SU001424 |
| Subject Type: | Yeast |
| Subject Species: | Saccharomyces cerevisiae |
| Taxonomy ID: | 4932 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001424 |
| Subject Type: | Yeast |
| Subject Species: | Saccharomyces cerevisiae |
| Taxonomy ID: | 4932 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Sample 86 | SA098034 | FL013801 | 1 | Timepoint |
| Sample 86 | SA098034 | FL013801 | 5 | Replicate |
Collection:
| Collection ID: | CO001419 |
| Collection Summary: | Samples were collected from fermentor eppendorf bioflo 120 through different timepoints of the Yeast Metabolic Cycle. Cells were snap frozen on liquid nitrogen for metabolite extraction. |
| Sample Type: | Yeast cells |
Treatment:
| Treatment ID: | TR001439 |
| Treatment Summary: | Cells were not treated, this was a timecourse on stable conditions, that yeast cells use to enter an ultradian rythm, which was measured during the timecourse. |
Sample Preparation:
| Sampleprep ID: | SP001432 |
| Sampleprep Summary: | Cell pellets were lyophilized to dryness overnight. The lyophilized pellet was homogenized in 50:50 acetonitrile/water with 0.3 % formic acid for OAs and AAs, 5% TCA for acetyl and malonyl CoA, 0.5M PCA for oxidized nucleotides and 50:50 methanol/NaOH for reduced nucleotides using the Precellys bead-based homogenizing system. Precellys tough micro-organism lysing kit (VK-05) was used to burst the yeast cells. An aliquot of homogenate for the required assay was aliquoted and immediately stored at -80oC. |
Combined analysis:
| Analysis ID | AN002246 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Hypercarb |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Thermo Quantiva |
| Ion Mode | POSITIVE |
| Units | nmoles/mg protein |
Chromatography:
| Chromatography ID: | CH001651 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Hypercarb |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002092 |
| Analysis ID: | AN002246 |
| Instrument Name: | Thermo Quantiva |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | The raw data was processed using Xcalibur 3.0. |
| Ion Mode: | POSITIVE |
