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MB Sample ID: SA098038

Local Sample ID:Sample 83
Subject ID:SU001424
Subject Type:Yeast
Subject Species:Saccharomyces cerevisiae
Taxonomy ID:4932

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Subject:

Subject ID:SU001424
Subject Type:Yeast
Subject Species:Saccharomyces cerevisiae
Taxonomy ID:4932

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
Sample 83SA098038FL01380520Timepoint
Sample 83SA098038FL0138054Replicate

Collection:

Collection ID:CO001419
Collection Summary:Samples were collected from fermentor eppendorf bioflo 120 through different timepoints of the Yeast Metabolic Cycle. Cells were snap frozen on liquid nitrogen for metabolite extraction.
Sample Type:Yeast cells

Treatment:

Treatment ID:TR001439
Treatment Summary:Cells were not treated, this was a timecourse on stable conditions, that yeast cells use to enter an ultradian rythm, which was measured during the timecourse.

Sample Preparation:

Sampleprep ID:SP001432
Sampleprep Summary:Cell pellets were lyophilized to dryness overnight. The lyophilized pellet was homogenized in 50:50 acetonitrile/water with 0.3 % formic acid for OAs and AAs, 5% TCA for acetyl and malonyl CoA, 0.5M PCA for oxidized nucleotides and 50:50 methanol/NaOH for reduced nucleotides using the Precellys bead-based homogenizing system. Precellys tough micro-organism lysing kit (VK-05) was used to burst the yeast cells. An aliquot of homogenate for the required assay was aliquoted and immediately stored at -80oC.

Combined analysis:

Analysis ID AN002246
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Dionex Ultimate 3000
Column Hypercarb
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Thermo Quantiva
Ion Mode POSITIVE
Units nmoles/mg protein

Chromatography:

Chromatography ID:CH001651
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Hypercarb
Chromatography Type:Reversed phase

MS:

MS ID:MS002092
Analysis ID:AN002246
Instrument Name:Thermo Quantiva
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:The raw data was processed using Xcalibur 3.0.
Ion Mode:POSITIVE
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