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MB Sample ID: SA098087
| Local Sample ID: | Mut _ML_6 |
| Subject ID: | SU001425 |
| Subject Type: | Bacteria |
| Subject Species: | Escherichia coli |
| Taxonomy ID: | 562 |
| Genotype Strain: | ATCC 25922 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001425 |
| Subject Type: | Bacteria |
| Subject Species: | Escherichia coli |
| Taxonomy ID: | 562 |
| Genotype Strain: | ATCC 25922 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Mut _ML_6 | SA098087 | FL013854 | Mutant | Genotype |
| Mut _ML_6 | SA098087 | FL013854 | Mid Log | Phase |
Collection:
| Collection ID: | CO001420 |
| Collection Summary: | Samples were collected in the mid log and stationary phases, washed with cold PBS, frozen at -80 ºC. Please see attached PDF for detailed procedures. |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR001440 |
| Treatment Summary: | The samples were not treated with anything. I collected the samples then extracted the hydrophilic metabolites, placed them in an NMR buffer then ran them on the NMR. All these procedures were uploaded. |
Sample Preparation:
| Sampleprep ID: | SP001433 |
| Sampleprep Summary: | The hydrophilic metabolites were extracted, the samples were added to an NMR buffer, run on the NMR and profiled, standardized to their concentrations and analyzed. See uploaded protocol for more details. |
Analysis:
| MB Sample ID: | SA098087 |
| Analysis ID: | AN002247 |
| Analysis Type: | NMR |
| Num Factors: | 4 |
| Num Metabolites: | 31 |
| Units: | mM |
NMR:
| NMR ID: | NM000162 |
| Analysis ID: | AN002247 |
| Instrument Name: | Buker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Spectrometer Frequency: | 600 MHz |
