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MB Sample ID: SA099076
| Local Sample ID: | VV_104_HEpG2_IPA_3 |
| Subject ID: | SU001437 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001437 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| VV_104_HEpG2_IPA_3 | SA099076 | FL014043 | IPA | Solvent |
Collection:
| Collection ID: | CO001432 |
| Collection Summary: | Lipids were extracted either with a biphasic extraction with CHCl3 or MTBE, or with monophasic extractions of IPA or a mixture 1:1 of butanol-methanol (BuMe) |
| Sample Type: | HepG2 cells |
Treatment:
| Treatment ID: | TR001452 |
| Treatment Summary: | In this experiment we are not treating cells with any drug. In contrast, we are evaluating extraction efficiency of different solvents. |
Sample Preparation:
| Sampleprep ID: | SP001445 |
| Sampleprep Summary: | Monophasic extraction straight from cell plates using BuMe or IPA. |
Combined analysis:
| Analysis ID | AN002269 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | Normalised peak area |
Chromatography:
| Chromatography ID: | CH001668 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002113 |
| Analysis ID: | AN002269 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | See Methods file. |
| Ion Mode: | POSITIVE |
