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MB Sample ID: SA099077
Local Sample ID: | VV_103_HEpG2_IPA_2 |
Subject ID: | SU001437 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001437 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
VV_103_HEpG2_IPA_2 | SA099077 | FL014043 | IPA | Solvent |
Collection:
Collection ID: | CO001432 |
Collection Summary: | Lipids were extracted either with a biphasic extraction with CHCl3 or MTBE, or with monophasic extractions of IPA or a mixture 1:1 of butanol-methanol (BuMe) |
Sample Type: | HepG2 cells |
Treatment:
Treatment ID: | TR001452 |
Treatment Summary: | In this experiment we are not treating cells with any drug. In contrast, we are evaluating extraction efficiency of different solvents. |
Sample Preparation:
Sampleprep ID: | SP001445 |
Sampleprep Summary: | Monophasic extraction straight from cell plates using BuMe or IPA. |
Combined analysis:
Analysis ID | AN002269 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 |
Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | Normalised peak area |
Chromatography:
Chromatography ID: | CH001668 |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS002113 |
Analysis ID: | AN002269 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | See Methods file. |
Ion Mode: | POSITIVE |