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MB Sample ID: SA099127
| Local Sample ID: | 27_28 |
| Subject ID: | SU001438 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001438 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 27_28 | SA099127 | FL014051 | ICM | Condition |
| 27_28 | SA099127 | FL014051 | M | Gender |
Collection:
| Collection ID: | CO001433 |
| Collection Summary: | Donor hearts were procured but not used for heart transplantation (reasons included transportation logistics, donor-recipient mismatch in size, and immune incompatibility), whilst heart failure samples comprised those patients undergoing heart transplantation. |
| Sample Type: | Heart |
Treatment:
| Treatment ID: | TR001453 |
| Treatment Summary: | LV samples from both donors and heart failure patients were collected and snap frozen (-196 °C) immediately within the operating theatre. All samples were stored in the Sydney Heart Bank at -170 to -180°C from the time of harvest. |
Sample Preparation:
| Sampleprep ID: | SP001446 |
| Sampleprep Summary: | These samples are not post-mortem. Frozen heart tissues were crushed to powder in liquid nitrogen and weighed for metabolomic (~150 mg) analysis. |
Combined analysis:
| Analysis ID | AN002270 | AN002271 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1260 | Agilent 1260 |
| Column | Waters Atlantis HILIC (100 x 2.1mm,3um,100Å) | Waters XBridge Amide (100 x 4.6mm,3.5um) |
| MS Type | API | API |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | intensity (arbitrary unit) | intensity (arbitrary unit) |
Chromatography:
| Chromatography ID: | CH001669 |
| Instrument Name: | Agilent 1260 |
| Column Name: | Waters Atlantis HILIC (100 x 2.1mm,3um,100Å) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001670 |
| Instrument Name: | Agilent 1260 |
| Column Name: | Waters XBridge Amide (100 x 4.6mm,3.5um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS002114 |
| Analysis ID: | AN002270 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | API |
| MS Comments: | Interface: Turboionspray, positive ionization mode Scan Mode: Scheduled multiple reaction monitoring (MRM) sMRM Window: 30 sec Target Scan Time: 1.0 sec Source Temp: 450°C Ion Source position: Vertical: 1, Horizontal: 5 |
| Ion Mode: | POSITIVE |
| MS ID: | MS002115 |
| Analysis ID: | AN002271 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | API |
| MS Comments: | Interface: Turboionspray, negative ionization mode Scan Mode: Scheduled multiple reaction monitoring (MRM) sMRM Window: 30 sec Target Scan Time: 1.0 sec Source Temp: 450°C Ion Source position: Vertical: 1, Horizontal: 5 |
| Ion Mode: | NEGATIVE |
