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MB Sample ID: SA100904
Local Sample ID: | Sample_5 |
Subject ID: | SU001454 |
Subject Type: | Other |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001454 |
Subject Type: | Other |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Sample_5 | SA100904 | FL014195 | Serum | Treatment |
Collection:
Collection ID: | CO001449 |
Collection Summary: | FI-MS method optimization was performed with commercially available serum, Human AB Serum (Biological Industries USA, Inc., USA). Matrix effect experiments and biological applications were performed with 98 serum samples of healthy individuals obtained from Rambam Hospital, Haifa, Israel (IRB 0481-18-RMB). |
Sample Type: | Blood (serum) |
Treatment:
Treatment ID: | TR001469 |
Treatment Summary: | To extract metabolites and lipids from serum samples, we mixed 20 µL of serum with an extraction solution for metabolomics analysis and 10 µL for lipidomics in 96-deep well plates. For lipidomics analysis, we utilized 100 µL of 2-propanol/methanol (6:1, v/v); and for metabolomics analysis, 100 µL of methanol/acetonitrile/water (5:3:1, v/v/v). After 10 min of vortexing, 800 rpm, precipitated proteins were separated by centrifugation for 20 min at 4 °C and 4000 rcf; supernatants were stored at -80 °C prior the analysis. |
Sample Preparation:
Sampleprep ID: | SP001462 |
Sampleprep Summary: | To extract metabolites and lipids from serum samples, we mixed 20 µL of serum with an extraction solution for metabolomics analysis and 10 µL for lipidomics in 96-deep well plates. For lipidomics analysis, we utilized 100 µL of 2-propanol/methanol (6:1, v/v); and for metabolomics analysis, 100 µL of methanol/acetonitrile/water (5:3:1, v/v/v). After 10 min of vortexing, 800 rpm, precipitated proteins were separated by centrifugation for 20 min at 4 °C and 4000 rcf; supernatants were stored at -80 °C prior the analysis. |
Combined analysis:
Analysis ID | AN002300 |
---|---|
Analysis type | MS |
Chromatography type | None (Direct infusion) |
Chromatography system | none |
Column | none |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | UNSPECIFIED |
Units | intensity |
Chromatography:
Chromatography ID: | CH001690 |
Instrument Name: | none |
Column Name: | none |
Chromatography Type: | None (Direct infusion) |
MS:
MS ID: | MS002143 |
Analysis ID: | AN002300 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | To determine the number of reproducibly detected m/z features by a specific FI-MS method configuration, we performed 6 repeated injections of the biological sample from the same vial followed by the injection of 6 blank samples (i.e. sample preparation protocol applied to a water sample) and identified reproducibly detected features based on low RSD (<30%) and high SNR (>4) |
Ion Mode: | UNSPECIFIED |