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MB Sample ID: SA106751
| Local Sample ID: | 7228773 |
| Subject ID: | SU001460 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001460 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 7228773 | SA106751 | FL014231 | Female | Sex |
| 7228773 | SA106751 | FL014231 | 6 | cc |
Collection:
| Collection ID: | CO001455 |
| Collection Summary: | Plasma was obtained from human whole blood every 3 months up until 48 months; after 48 months, plasma was collected every 3 to 6 months depending on antibody status. |
| Sample Type: | Plasma |
Treatment:
| Treatment ID: | TR001475 |
| Treatment Summary: | n/a |
Sample Preparation:
| Sampleprep ID: | SP001468 |
| Sampleprep Summary: | Blood plasma or serum is extracted following the protocols published e.g. in Fiehn et al. PLoS ONE (2010) e15234. 30 ?L aliquots are extracted by 1 mL of degassed acetonitrile : isopropanol : water (3:3:2,v/v/v ) at –20°C, centrifuged and decanted with subsequent evaporation of the solvent to complete dryness. A clean-up step with acetonitrile/water (1:1) removes membrane lipids and triglycerides. The cleaned extract is aliquoted into two equal portions and the supernatant is dried down again. Internal standards C08-C30 FAMEs are added and the sample is derivatized by methoxyamine hydrochloride in pyridine and subsequently by N-methyl-N-trimethylsilyltrifluoroacetamide for trimethylsilylation of acidic protons. |
Combined analysis:
| Analysis ID | AN002314 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Leco Pegasus IV GC |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus IV TOF |
| Ion Mode | UNSPECIFIED |
| Units | peak heights |
Chromatography:
| Chromatography ID: | CH001701 |
| Instrument Name: | Leco Pegasus IV GC |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS002157 |
| Analysis ID: | AN002314 |
| Instrument Name: | Leco Pegasus IV TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| MS Comments: | See protocol. |
| Ion Mode: | UNSPECIFIED |
