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MB Sample ID: SA113202
| Local Sample ID: | 224_001 |
| Subject ID: | SU001464 |
| Subject Type: | Mammal |
| Subject Species: | Sus scrofa |
| Taxonomy ID: | 9823 |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001464 |
| Subject Type: | Mammal |
| Subject Species: | Sus scrofa |
| Taxonomy ID: | 9823 |
| Gender: | Male |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 224_001 | SA113202 | FL014254 | 12 | Timepoint |
| 224_001 | SA113202 | FL014254 | 12 | Timepoint |
Collection:
| Collection ID: | CO001459 |
| Collection Summary: | Whole blood (WB) samples were collected from the indwelling line in the internal jugular vein. Blood was collected into 4ml Vacutainer tubes containing sodium heparin. Following collection, tubes were inverted several times to ensure adequate mixing and were immediately placed on ice. Each of two 600uL aliquots were added to screw-top cryogenic storage tubes and flash frozen in liquid nitrogen. Frozen samples were placed on dry ice until the completion of the experiment at which time they were transferred to storage in liquid nitrogen and stored until the time of assay. The remaining whole blood volume in the collection tube was centrifuged (1300g for 10 min at 4C). Aliquots of plasma(~600uL) were transferred into screw-top cryogenic storage tubes, flash frozen in liquid nitrogen, placed on dry ice, and stored in liquid nitrogen for future assays. |
| Sample Type: | Blood (whole) |
| Storage Conditions: | Described in summary |
Treatment:
| Treatment ID: | TR001479 |
| Treatment Summary: | Male swine (n=5) were anesthetized, mechanically ventilated, and surgically instrumented for continuous hemodynamic monitoring and serial blood sampling. Animals were inoculated with uropathogenic E. coli by direct injection into the renal parenchyma and were maintained under anesthesia for up to 24 hours. The natural history of the infection was studied, animals were not resuscitated. Multi-dimensional data were collected hourly to every 6 hours; all animals were euthanized when at predetermined physiologic endpoints. |
Sample Preparation:
| Sampleprep ID: | SP001472 |
| Sampleprep Summary: | Whole blood samples were thawed on ice and subjected to a methanol-chloroform precipitation. Briefly, samples were thawed in an ice-water bath after which 500 μL of blood was transferred to a microcentrifuge tube and 1ml of a 1:1 methanol-chloroform solution was added. Samples were sonicated for 2 minutes at 4°C, then incubated at -20°C for 20 minutes, and then centrifuged (13,400g at 4°C for 30 minutes). The aqueous supernatant was transferred to a new microcentrifuge tube and dried by lyophilization. Samples were then resuspended in 600 μL of 50mM sodium phosphate buffer in D2O for NMR analysis. |
| Processing Storage Conditions: | On ice |
Analysis:
| MB Sample ID: | SA113202 |
| Analysis ID: | AN002319 |
| Analysis Type: | NMR |
| Num Factors: | 3 |
| Num Metabolites: | 39 |
| Units: | uM |
NMR:
| NMR ID: | NM000165 |
| Analysis ID: | AN002319 |
| Instrument Name: | Varian |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Spectrometer Frequency: | 500 MHz |
