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MB Sample ID: SA115963
| Local Sample ID: | Adult5 |
| Subject ID: | SU001487 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | newborn mice of 24hr old or adult mice of 13 week old |
| Gender: | Male |
| Animal Animal Supplier: | Charles River Laboratories (Wilmington, MA) |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001487 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | newborn mice of 24hr old or adult mice of 13 week old |
| Gender: | Male |
| Animal Animal Supplier: | Charles River Laboratories (Wilmington, MA) |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Adult5 | SA115963 | FL014432 | Control | Condition |
| Adult5 | SA115963 | FL014432 | 13 weeks | Age |
Collection:
| Collection ID: | CO001482 |
| Collection Summary: | Metabolic profiling on whole homogenate brain tissues collected from 24 hours or 13 weeks’ old mice was carried out at the West Coast metabolics center (University of California Davis). |
| Sample Type: | Brain |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001502 |
| Treatment Summary: | Swiss Webster mice, 8-9 weeks’ age, were obtained from Charles River Laboratories (Wilmington, MA). One male and two female mice were group-housed and maintained on a 12- hours light/dark cycle (light on at 7:00 am) with food and water available ad libitum. Pregnant mice were individually housed from the thirteen day of pregnancy (gestational day 13) until delivery. Pregnant mice that mated with the same male mouse were subcutaneously administered with L-Methionine (MET) at a dose of 750 mg/kg (Sigma-Aldrich) or saline (SAL) twice a day for seven to eight consecutive days from gestational day 14 until delivery. |
Sample Preparation:
| Sampleprep ID: | SP001495 |
| Sampleprep Summary: | Metabolic profiling on whole homogenate brain tissues collected from 24 hours or 13 weeks’ old mice was carried out at the West Coast metabolics center (University of California Davis). |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN002363 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | SCIEX 6600TTOF |
| Column | Thermo Accucore HILIC (100 x 2.1mm,2.6um) |
| MS Type | APCI |
| MS instrument type | Triple TOF |
| MS instrument name | ABI Sciex 6600 TripleTOF |
| Ion Mode | POSITIVE |
| Units | m/z |
Chromatography:
| Chromatography ID: | CH001733 |
| Instrument Name: | SCIEX 6600TTOF |
| Column Name: | Thermo Accucore HILIC (100 x 2.1mm,2.6um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS002205 |
| Analysis ID: | AN002363 |
| Instrument Name: | ABI Sciex 6600 TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | APCI |
| MS Comments: | None |
| Ion Mode: | POSITIVE |
