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MB Sample ID: SA120089
| Local Sample ID: | Pcl_BHI_3 |
| Subject ID: | SU001500 |
| Subject Type: | Bacteria |
| Subject Species: | Staphylococcus epidermidis |
| Taxonomy ID: | 1282 |
| Genotype Strain: | ATCC 12600; ATCC 12228 |
| Cell Biosource Or Supplier: | ATCC |
| Cell Strain Details: | ATCC 12600; ATCC 12228 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001500 |
| Subject Type: | Bacteria |
| Subject Species: | Staphylococcus epidermidis |
| Taxonomy ID: | 1282 |
| Genotype Strain: | ATCC 12600; ATCC 12228 |
| Cell Biosource Or Supplier: | ATCC |
| Cell Strain Details: | ATCC 12600; ATCC 12228 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Pcl_BHI_3 | SA120089 | FL014864 | MEDIUM | Treatment |
| Pcl_BHI_3 | SA120089 | FL014864 | BHI | Culture Medium |
Collection:
| Collection ID: | CO001495 |
| Collection Summary: | Staphylococcus aureus (ATCC 12600) and Staphylococcus epidermidis (ATCC 12228) were cultured in four filter-sterilized complex media for metabolomics analysis: Brain Heart Infusion broth (BHI; Bacto); lysogeny broth Lennox (LB; Fisher Scientific); Mueller Hinton broth (MHB; Difco); and Tryptic Soy broth (TSB; Bacto). Each species was prepared in biological triplicate. Uninoculated media control blanks were prepared in six replicates for BHI, LB, and MHB, and in triplicate for TSB, following the same procedures as the bacterial samples and processed in parallel. Samples were transferred to capped GC vials and stored at -20 °C for approximately two weeks prior to GC×GC-TOFMS analysis. |
| Collection Protocol Filename: | CLJenkins_Collection_Protocol_Metadata.txt |
| Sample Type: | Bacterial cells |
| Collection Frequency: | Once, at the completion of 24 hour aerobic incubation at 37 C with orbital shaking |
Treatment:
| Treatment ID: | TR001515 |
| Treatment Summary: | Two ATCC strains of staphylococci were grown in four different filter-sterilized complex media under identical conditions. Please see the Collection Protocol for details. |
| Cell Media: | BHI Broth, LB Lennox, Mueller Hinton Broth, Tryptic Soy Broth |
Sample Preparation:
| Sampleprep ID: | SP001508 |
| Sampleprep Summary: | No sample preparation was required due to sampling via Head-Space Solid-phase microextraction by autosampler robot. |
| Sampleprep Protocol Filename: | CLJenkins_MS__Methods.pdf |
Combined analysis:
| Analysis ID | AN002384 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890B |
| Column | Multidimensional configuration |
| MS Type | EI |
| MS instrument type | GC x GC-TOF |
| MS instrument name | Leco Pegasus 4D GCxGC TOF |
| Ion Mode | POSITIVE |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH001751 |
| Chromatography Summary: | Comprehensive two-dimensional gas chromatography with multi-dimensional column configuration |
| Instrument Name: | Agilent 7890B |
| Column Name: | Multidimensional configuration |
| Chromatography Type: | GC |
MS:
| MS ID: | MS002226 |
| Analysis ID: | AN002384 |
| Instrument Name: | Leco Pegasus 4D GCxGC TOF |
| Instrument Type: | GC x GC-TOF |
| MS Type: | EI |
| MS Comments: | none |
| Ion Mode: | POSITIVE |
