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MB Sample ID: SA121962
| Local Sample ID: | 217_pos |
| Subject ID: | SU001509 |
| Subject Type: | Plant |
| Subject Species: | Acer saccharum |
| Taxonomy ID: | 4024 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001509 |
| Subject Type: | Plant |
| Subject Species: | Acer saccharum |
| Taxonomy ID: | 4024 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 217_pos | SA121962 | FL014915 | Ottawa Valley District | OMSPA region |
Collection:
| Collection ID: | CO001504 |
| Collection Summary: | Sap samples (n = 282) were collected as approximately 10 ml aliquots into 15 ml plastic centrifuge tubes contain 1.5 mg of sodium benzoate and stored at 4 ºC. Samples were diluted 1:3 in methanol containing 0.15% formic acid and 30 mM ammonium formate |
| Sample Type: | Plant |
| Storage Conditions: | 4℃ |
Treatment:
| Treatment ID: | TR001524 |
| Treatment Summary: | In 2019, maple sap and syrup samples were collected over the entire production season by 12 members of the Ontario Maple Syrup Producers Association (OMSPA). Sap samples were collected into tubes containing 1.5 mg sodium benzoate. |
Sample Preparation:
| Sampleprep ID: | SP001517 |
| Sampleprep Summary: | Samples were diluted 1:3 with methanol containing 0.15% FA and 30mM ammonium formate |
Combined analysis:
| Analysis ID | AN002398 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1290 |
| Column | Agilent HILIC-Z (2.1 × 100 mm, 2.7 µm) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | concentrations (ug/ml) |
Chromatography:
| Chromatography ID: | CH001762 |
| Instrument Name: | Agilent 1290 |
| Column Name: | Agilent HILIC-Z (2.1 × 100 mm, 2.7 µm) |
| Column Temperature: | 35 |
| Flow Gradient: | 0 min, 100% B; 0.5 min, 100% B; 5.3 min, 80% B; 9.5 min, 30% B; 13.5 min, 30% B, 14.5 min 100% B and 16.5 min, 100% B. |
| Flow Rate: | 0.350uL min01 |
| Solvent A: | 100% water; 20 mM ammonium formate |
| Solvent B: | 90% acetonitrile/10% water; 20 mM ammonium formate |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS002239 |
| Analysis ID: | AN002398 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The HILIC acquisition mode was a top 3 data-dependent acquisition (DDA) experiment comprised of a full MS scan in the mass range of m/z 58-870 at 35,000 resolution, followed by MS/MS scans at 17,500 resolution, isolation window of m/z 1.2 and collision energy of 28 |
| Ion Mode: | POSITIVE |
