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MB Sample ID: SA122053
| Local Sample ID: | 12A02 |
| Subject ID: | SU001511 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001511 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 12A02 | SA122053 | FL014925 | Pre | Group |
Collection:
| Collection ID: | CO001506 |
| Collection Summary: | EBC from 11 CF patients with abnormalities in glucose homeostasis (as defined by having prediabetes/impaired glucose tolerance [CF IGT] on an oral glucose tolerance test) was collected both fasting and 2 hours following ingestion of a glucose drink. They were termed as “Pre” and “Post”, respectively. Of these 11 subjects, one did not have an adequate sample for the “Pre” and another did not have a sample for the “Post” study giving a total of 20 samples analyzed (one of then failed to purduce enough features as others and was regarded as an outlier and removed). EBC sample collection followed the guidelines approved by the Georgia Institute of Technology and the Emory University Institutional Review Boards (approval number IRB00000372). Samples were collected with an R-Tube collector (Respiratory Research, Inc., Austin, TX, USA). After collection, samples were immediately frozen at -80℃ until processed. |
| Sample Type: | Exhaled Breath condensate |
Treatment:
| Treatment ID: | TR001526 |
| Treatment Summary: | A total of 20 EBC samples (10 for each group), each 50 µL in volume before concentration, were phenotyped. A pooled sample, which was used as a QC, was prepared by taking 5 µL from each EBC sample and mixing the aliquots together. Then, the 20 samples, together with the pooled QC sample and the blank sample (containing only ultrapure water) were lyophilized at -40 C and 100 mTorr for 24h in a VirTis Benchtop freeze-drier (LP Industries, Stone Ridge, NY, USA). Residues were then reconstituted in 9 μL of methanol/water 1:9 (v:v) with 1×10-6 M 13C-tyrosine spiked in. This resulted in a 5-fold concentration factor. |
Sample Preparation:
| Sampleprep ID: | SP001519 |
| Sampleprep Summary: | A total of 20 EBC samples (10 for each group), each 50 µL in volume before concentration, were phenotyped. A pooled sample, which was used as a QC, was prepared by taking 5 µL from each EBC sample and mixing the aliquots together. Then, the 20 samples, together with the pooled QC sample and the blank sample (containing only ultrapure water) were lyophilized at -40 C and 100 mTorr for 24h in a VirTis Benchtop freeze-drier (LP Industries, Stone Ridge, NY, USA). Residues were then reconstituted in 9 μL of methanol/water 1:9 (v:v) with 1×10-6 M 13C-tyrosine spiked in. This resulted in a 5-fold concentration factor. |
Combined analysis:
| Analysis ID | AN002401 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | none |
| Column | none |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Synapt G2 S QTOF |
| Ion Mode | NEGATIVE |
| Units | Normalized Intensity (Intensity ratio against internal standard signal)) |
Chromatography:
| Chromatography ID: | CH001764 |
| Instrument Name: | none |
| Column Name: | none |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS002242 |
| Analysis ID: | AN002401 |
| Instrument Name: | Waters Synapt G2 S QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Negative ion mode in the 50-750 m/z range was used for experiments Detailed data process see attached Method file |
| Ion Mode: | NEGATIVE |
