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MB Sample ID: SA122104
| Local Sample ID: | CSI-DAO1 |
| Subject ID: | SU001513 |
| Subject Type: | Bacteria |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | 8 weeks of age |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001513 |
| Subject Type: | Bacteria |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | 8 weeks of age |
| Gender: | Male |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| CSI-DAO1 | SA122104 | FL014931 | DAOG181R | Genotype |
Collection:
| Collection ID: | CO001508 |
| Collection Summary: | Mice were euthanized and collect half length of small intestine (ileum). Contents of small intestine were mechanically squeezed by pipet tip into 1.5 mL tube and then flash-frozen in liquid nitrogen. |
| Sample Type: | contents of small intestine |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001528 |
| Treatment Summary: | Mice were generated by in vitro fertilization and transplantation to surrogate mothers, and pups C-sectioned on E20 were fostered to CD-1 mother with strain-selected microbiota in the animal facility of CIEA. |
Sample Preparation:
| Sampleprep ID: | SP001521 |
| Sampleprep Summary: | For extracting ionic metabolites. approximately 20 mg of contents of small intestine were dissolved in H2O containing internal standards (H3304-1002, HMT, Japan) with ratio of 1:9. After centrifugation, the supernatant was the centrifugically filtered through a Milipore 5000-Da cutoff filter to remove macromolecules for subsequent analysis with CE-TOFMS. For extracting non-ionic metabolites, approximately 20 mg of contents of small intestine were dissolved in 1 mL of methanol containing internal standard (H3304-1002, HMT). After centrifugation, 1o uL of the supernatant was transferred into grass vials for evaporation under nitrogen gas and reconstituted with 200 uL of 50% isopropanol for subsequent analysis with LC-TOFMS. |
Combined analysis:
| Analysis ID | AN002403 | AN002404 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | None (Direct infusion) | None (Direct infusion) |
| Chromatography system | none | none |
| Column | none | none |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6210 TOF | Agilent 6230 TOF |
| Ion Mode | UNSPECIFIED | UNSPECIFIED |
| Units | relative_area | relative_area |
Chromatography:
| Chromatography ID: | CH001766 |
| Instrument Name: | none |
| Column Name: | none |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS002244 |
| Analysis ID: | AN002403 |
| Instrument Name: | Agilent 6210 TOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | System control: Agilent G2201AA ChemStation software version B .03.01 Peaks were extracted using automatic integration software MasterHands to obtain peal information including m/z, peak area and migration time. Detected metabolites were plotted on metabolic pathway maps using VANTED software. |
| Ion Mode: | UNSPECIFIED |
| MS ID: | MS002245 |
| Analysis ID: | AN002404 |
| Instrument Name: | Agilent 6230 TOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | System control: Agilent G2201AA ChemStation software version B .03.01 Peaks were extracted using automatic integration software MasterHands to obtain peal information including m/z, peak area and retention time. Detected metabolites were plotted on metabolic pathway maps using VANTED software. |
| Ion Mode: | UNSPECIFIED |
