Return to study ST001609 main page
MB Sample ID: SA136724
Local Sample ID: | BY4742-12 |
Subject ID: | SU001686 |
Subject Type: | Yeast |
Subject Species: | Saccharomyces cerevisiae |
Taxonomy ID: | 4932 |
Genotype Strain: | BY4742, YGR023w |
Cell Biosource Or Supplier: | Open Biosystems |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001686 |
Subject Type: | Yeast |
Subject Species: | Saccharomyces cerevisiae |
Taxonomy ID: | 4932 |
Genotype Strain: | BY4742, YGR023w |
Cell Biosource Or Supplier: | Open Biosystems |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
BY4742-12 | SA136724 | FL017497 | Wild-type | Strain |
Collection:
Collection ID: | CO001679 |
Collection Summary: | Cells were grown at 27°C, 210 rpm in 5 mL of complete synthetic medium (CSM, 0.67% Nitrogen base without amino acids and ammonium sulfate, 2% glucose). The next day, cells were replaced with fresh CSM and grow at 27°C to reach an OD of 0.6 - 0.7. 25 ml of each sample were harvested by centrifugation at 3,838 x g for 5 min at 4°C, washed with 1 ml ice cold sterile water, quenched using ice cold methanol, and stored at -80°C. |
Sample Type: | Yeast cells |
Treatment:
Treatment ID: | TR001699 |
Treatment Summary: | No treatment |
Sample Preparation:
Sampleprep ID: | SP001692 |
Sampleprep Summary: | Extraction of metabolites was performed by homogenization in 1 mL of cold methanol/H2O (1:1) extraction solution and centrifugated at 167 x g at 4°C for 5 min. Supernatants were collected and evaporated to dryness in a nitrogen stream stream at 50 ºC (RapidVap, Labconco). Nitrilation was performed by adding 150 μL of 0.2 mM hydroxylammonium chloride in pyridine to the dried sample, and then heating at 90ºC for 40 minutes. After that, acetylation was performed by adding 250 μL of acetic anhydride, and then heating at 90 ºC for 60 minutes. After that, the sample was dried again in a nitrogen gas stream, and then redissolved in 400 μL of ethyl acetate. Derivatized samples were collected and stored at -20ºC. |
Combined analysis:
Analysis ID | AN002643 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Shimadzu GCMS-QP2010 ultra |
Column | Shimadzu SH-RXI (30m x 0.25mm,0.25um) |
MS Type | EI |
MS instrument type | Single quadrupole |
MS instrument name | Shimadzu QP2010 Ultra |
Ion Mode | POSITIVE |
Units | mM |
Chromatography:
Chromatography ID: | CH001952 |
Instrument Name: | Shimadzu GCMS-QP2010 ultra |
Column Name: | Shimadzu SH-RXI (30m x 0.25mm,0.25um) |
Chromatography Type: | GC |
MS:
MS ID: | MS002455 |
Analysis ID: | AN002643 |
Instrument Name: | Shimadzu QP2010 Ultra |
Instrument Type: | Single quadrupole |
MS Type: | EI |
MS Comments: | Inlet temperature was 280°C; ion source temperature was 200 °C. MS conditions were set as follows: SIM acquisition mode, electron energy of 70 eV, quadrupole scan range of m/z 115; 187; 212;314. Data were processed using the GCMS Solution Postrun Analysis software (Shimadzu Inc) for metabolites identification from their electron impact mass spectra by comparison to the NIST 2014 spectral mass library. |
Ion Mode: | POSITIVE |