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MB Sample ID: SA136784
Local Sample ID: | YGR023w-7 |
Subject ID: | SU001689 |
Subject Type: | Yeast |
Subject Species: | Saccharomyces cerevisiae |
Taxonomy ID: | 4932 |
Genotype Strain: | BY4742, YGR023w |
Cell Biosource Or Supplier: | Open Biosystems |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001689 |
Subject Type: | Yeast |
Subject Species: | Saccharomyces cerevisiae |
Taxonomy ID: | 4932 |
Genotype Strain: | BY4742, YGR023w |
Cell Biosource Or Supplier: | Open Biosystems |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
YGR023w-7 | SA136784 | FL017504 | mtl1Δ | Strain |
Collection:
Collection ID: | CO001682 |
Collection Summary: | Cells were grown at 27°C, 210 rpm in 5 mL of complete synthetic medium (CSM, 0.67% Nitrogen base without amino acids and ammonium sulfate, 2% glucose). The next day, cells were replaced with fresh CSM and grow at 27°C to reach an OD of 0.6 - 0.7. 25 ml of each sample were harvested by centrifugation at 3,838 x g for 5 min at 4°C, washed with 1 ml ice cold sterile water, quenched using ice cold methanol, and stored at -80°C. |
Sample Type: | Yeast cells |
Treatment:
Treatment ID: | TR001702 |
Treatment Summary: | No treatment |
Sample Preparation:
Sampleprep ID: | SP001695 |
Sampleprep Summary: | Extraction of metabolites was performed by homogenization in 1 mL of cold methanol/H2O (1:1) extraction solution and centrifugated at 167 x g at 4°C for 5 min. Supernatants were collected and evaporated to dryness in a nitrogen stream stream at 50 ºC (RapidVap, Labconco), and derivatized by methoxyamination by adding 50 μl of 20 mg/ml solution of methoxyamine hydrochloride in pyridine followed by incubation at 37°C for 2 hours. Trimethylsilylation was subsequently performed by adding 50 µl of N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA+1% TMCS) and incubated for 1 hour at 65°C. Samples were centrifuged at 13000 rpm for 10 minutes at RT. Supernatants were transferred to glass vials for GC/MS analysis |
Combined analysis:
Analysis ID | AN002647 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Shimadzu GCMS-QP2010 ultra |
Column | Shimadzu SH-Rxi-5ms (30m x 0.25mm,0.25um) |
MS Type | EI |
MS instrument type | Single quadrupole |
MS instrument name | Shimadzu QP2010 Ultra |
Ion Mode | POSITIVE |
Units | mM |
Chromatography:
Chromatography ID: | CH001955 |
Instrument Name: | Shimadzu GCMS-QP2010 ultra |
Column Name: | Shimadzu SH-Rxi-5ms (30m x 0.25mm,0.25um) |
Internal Standard: | 1 mM 2-Fluobiphenyl |
Chromatography Type: | GC |
MS:
MS ID: | MS002459 |
Analysis ID: | AN002647 |
Instrument Name: | Shimadzu QP2010 Ultra |
Instrument Type: | Single quadrupole |
MS Type: | EI |
MS Comments: | Inlet temperature was 280 °C; ion source temperature was 200 °C. MS conditions were set as follows: full scan mode, electron energy of 70 eV, quadrupole scan range of m/z 35–700. Data were processed using the GCMS Solution Postrun Analysis software (Shimadzu Inc) for metabolites identification from their electron impact mass spectra by comparison to the NIST 2014 spectral mass library. |
Ion Mode: | POSITIVE |