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MB Sample ID: SA137915
| Local Sample ID: | C1 |
| Subject ID: | SU001698 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001698 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| C1 | SA137915 | FL017542 | D-HANKS | Treatment |
Collection:
| Collection ID: | CO001691 |
| Collection Summary: | At the 5th day of secondary organoid culture, the organoids were treated with fructose (10 mM D-fructose)-containing IntestiCult organoid growth medium for 24 hr and washed with pre-cooled PBS before harvesting. |
| Sample Type: | Intestine |
Treatment:
| Treatment ID: | TR001711 |
| Treatment Summary: | At the 5th day of secondary organoid culture, the organoids were treated with fructose (10 mM D-fructose)-containing IntestiCult organoid growth medium for 24 hr and washed with pre-cooled PBS before harvesting. |
Sample Preparation:
| Sampleprep ID: | SP001704 |
| Sampleprep Summary: | To harvest intracellular metabolites, organoid samples were washed with pre-cooled PBS for three times and metabolism was quenched with 80% ice-cold methanol. Pre-cooled methanol (1mL) with para-chlorophenylalanine (1 µM) as the internal standard was added to each sample and incubated on ice for 10 min. Sample lysates were then centrifuged at 4 °C for 15 min at 23,000 g. |
Combined analysis:
| Analysis ID | AN002656 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Waters Acquity H-Class |
| Column | Waters XBridge Amide (100 x 4.6mm,3.5um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Synapt G2 Si QTOF |
| Ion Mode | NEGATIVE |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH001959 |
| Instrument Name: | Waters Acquity H-Class |
| Column Name: | Waters XBridge Amide (100 x 4.6mm,3.5um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS002463 |
| Analysis ID: | AN002656 |
| Instrument Name: | Waters Synapt G2 Si QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Fast data dependent acquisition The Progenesis QI MetaboAnalyst 3.0 |
| Ion Mode: | NEGATIVE |
