Return to study ST001623 main page
MB Sample ID: SA137930
Local Sample ID: | C4 |
Subject ID: | SU001700 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Female |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001700 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Female |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
C4 | SA137930 | FL017546 | Con | Treatment |
Collection:
Collection ID: | CO001693 |
Collection Summary: | jejunum tissue (30 mg) was transferred to homogenizer (pre-cooled at -20 ℃) and 100 µL of H2O (pre-cooled at 4℃) was added for homogenate for 1 min. 1 μg/mL 4-chloro-phenylalanine and 800 µL methanol solution were added for protein precipitation and vortexed for 10 min. The sample lysates were centrifuged at 30000g for 10 min at 4 °C, and dried under vacuum. It was reconstituted with 100 μL of methanol: water solution (50:50, v/v) before analysis. |
Sample Type: | Intestine |
Treatment:
Treatment ID: | TR001713 |
Treatment Summary: | PD was supplemented with raffinose, a significant increase in intestinal fructose in stressed mice could be observed. |
Sample Preparation:
Sampleprep ID: | SP001706 |
Sampleprep Summary: | Briefly, tissue (30 mg) was transferred to homogenizer (pre-cooled at -20 ℃) and 100 µL of H2O (pre-cooled at 4℃) was added for homogenate for 1 min. 1 μg/mL 4-chloro-phenylalanine and 800 µL methanol solution were added for protein precipitation and vortexed for 10 min. The sample lysates were centrifuged at 30000g for 10 min at 4 °C, and dried under vacuum. |
Combined analysis:
Analysis ID | AN002658 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Waters Acquity H-Class |
Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Xevo G2-XS, Waters |
Ion Mode | NEGATIVE |
Units | relative peak area |
Chromatography:
Chromatography ID: | CH001961 |
Instrument Name: | Waters Acquity H-Class |
Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
Chromatography Type: | HILIC |
MS:
MS ID: | MS002465 |
Analysis ID: | AN002658 |
Instrument Name: | Xevo G2-XS, Waters |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | MS MassLynx v4.1 Quanlynx v4.1 |
Ion Mode: | NEGATIVE |