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MB Sample ID: SA137939
| Local Sample ID: | S3 |
| Subject ID: | SU001700 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001700 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| S3 | SA137939 | FL017548 | Stress | Treatment |
Collection:
| Collection ID: | CO001693 |
| Collection Summary: | jejunum tissue (30 mg) was transferred to homogenizer (pre-cooled at -20 ℃) and 100 µL of H2O (pre-cooled at 4℃) was added for homogenate for 1 min. 1 μg/mL 4-chloro-phenylalanine and 800 µL methanol solution were added for protein precipitation and vortexed for 10 min. The sample lysates were centrifuged at 30000g for 10 min at 4 °C, and dried under vacuum. It was reconstituted with 100 μL of methanol: water solution (50:50, v/v) before analysis. |
| Sample Type: | Intestine |
Treatment:
| Treatment ID: | TR001713 |
| Treatment Summary: | PD was supplemented with raffinose, a significant increase in intestinal fructose in stressed mice could be observed. |
Sample Preparation:
| Sampleprep ID: | SP001706 |
| Sampleprep Summary: | Briefly, tissue (30 mg) was transferred to homogenizer (pre-cooled at -20 ℃) and 100 µL of H2O (pre-cooled at 4℃) was added for homogenate for 1 min. 1 μg/mL 4-chloro-phenylalanine and 800 µL methanol solution were added for protein precipitation and vortexed for 10 min. The sample lysates were centrifuged at 30000g for 10 min at 4 °C, and dried under vacuum. |
Combined analysis:
| Analysis ID | AN002658 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Waters Acquity H-Class |
| Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Xevo G2-XS, Waters |
| Ion Mode | NEGATIVE |
| Units | relative peak area |
Chromatography:
| Chromatography ID: | CH001961 |
| Instrument Name: | Waters Acquity H-Class |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS002465 |
| Analysis ID: | AN002658 |
| Instrument Name: | Xevo G2-XS, Waters |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | MS MassLynx v4.1 Quanlynx v4.1 |
| Ion Mode: | NEGATIVE |
