Return to study ST001652 main page
MB Sample ID: SA151737
Local Sample ID: | 8_A6_DT1 |
Subject ID: | SU001729 |
Subject Type: | Cultured cells |
Subject Species: | Plasmodium falciparum |
Taxonomy ID: | 5833 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001729 |
Subject Type: | Cultured cells |
Subject Species: | Plasmodium falciparum |
Taxonomy ID: | 5833 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
8_A6_DT1 | SA151737 | FL017813 | Drug Resistant | Genotype |
8_A6_DT1 | SA151737 | FL017813 | DSM-1 | Treatment |
Collection:
Collection ID: | CO001722 |
Collection Summary: | Cells were pelleted and the media aspirated prior to methanol extraction |
Sample Type: | Plasmodium cells |
Treatment:
Treatment ID: | TR001742 |
Treatment Summary: | Trophozoite-stage Plasmodium infected RBCs were treated with 10x IC50 of either drug for 2.5 hours |
Sample Preparation:
Sampleprep ID: | SP001735 |
Sampleprep Summary: | 90% Methanol/10% water at 50:1 solvent/cell pellet followed by vortexing and clarification via centrifugation |
Processing Method: | Lysis |
Processing Storage Conditions: | 4℃ |
Extract Cleanup: | Clarification via centrifugation followed by nitrogen drying |
Extract Storage: | -80℃ |
Sample Resuspension: | 6X pellet volume of HPLC H2O |
Sample Spiking: | Internal Standard 13C4, 15N1-Aspartate used for sample preparation normalizer |
Combined analysis:
Analysis ID | AN002699 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Exactive Orbitrap Plus |
Column | Phenomenex Synergi Hydro RP (100 x 2mm,2.5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | NEGATIVE |
Units | intensity |
Chromatography:
Chromatography ID: | CH001991 |
Chromatography Summary: | Low pH polar |
Instrument Name: | Thermo Exactive Orbitrap Plus |
Column Name: | Phenomenex Synergi Hydro RP (100 x 2mm,2.5um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS002497 |
Analysis ID: | AN002699 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Proprietary analytical software for integration and peak picking |
Ion Mode: | NEGATIVE |