Return to study ST001668 main page
MB Sample ID: SA152356
| Local Sample ID: | P1 |
| Subject ID: | SU001745 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001745 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| P1 | SA152356 | FL017915 | D-Allulose | Diet |
Collection:
| Collection ID: | CO001738 |
| Collection Summary: | The livers were collected and then flash-frozen in liquid N2 |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR001758 |
| Treatment Summary: | Seven-week old male Wistar rats were fed AIN-93G-based diets with/without 3% D-allulose, ad libitum for 4 weeks. |
Sample Preparation:
| Sampleprep ID: | SP001751 |
| Sampleprep Summary: | For CE-TOFMS, The frozen tissue was plunged into 50% acetonitrile/Milli-Q water containing internal standards. The tissues were homogenized thrice, and then centrifuged. The upper aqueous layer was centrifugally filtered to remove the proteins. The filtrate was then centrifugally concentrated and re-suspended in 50 µL of Milli-Q water for CE-MS analysis. For LC-TOFMS, the frozen tissue was plunged into 1% formic acid/acetonitrile containing the internal standard solution. Tissue was homogenized thrice, and the mixture was yet again homogenized after adding Milli-Q water and then centrifuged. The supernatant was collected and 500 1% formic acid/acetonitrile and Milli-Q water were added to the precipitate. Homogenization and centrifugation were performed as described previously, and the supernatant was mixed with previously collected one. The mixed supernatant was filtrated to remove the proteins and phospholipids. The filtrate was then desiccated and dissolved in 200 µL of isopropanol/Milli-Q water for LC-MS analysis. |
Combined analysis:
| Analysis ID | AN002720 | AN002721 | AN002722 | AN002723 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | CE | CE | Reversed phase | Reversed phase |
| Chromatography system | Agilent CE | Agilent CE | Agilent 1200SL | Agilent 1200SL |
| Column | fused silica capillary (50 μm i.d. × 80 cm) | fused silica capillary (50 μm i.d. × 80 cm) | ODS (2x50mm,2um) | ODS (2x50mm,2um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | QTOF | QTOF | QTOF | QTOF |
| MS instrument name | Agilent CE-TOFMS | Agilent CE-TOFMS | Agilent 6230 TOF | Agilent 6230 TOF |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Relative Area | Relative Area | Relative Area | Relative Area |
Chromatography:
| Chromatography ID: | CH002008 |
| Instrument Name: | Agilent CE |
| Column Name: | fused silica capillary (50 μm i.d. × 80 cm) |
| Chromatography Type: | CE |
| Chromatography ID: | CH002009 |
| Instrument Name: | Agilent 1200SL |
| Column Name: | ODS (2x50mm,2um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002517 |
| Analysis ID: | AN002720 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | POSITIVE |
| MS ID: | MS002518 |
| Analysis ID: | AN002721 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | NEGATIVE |
| MS ID: | MS002519 |
| Analysis ID: | AN002722 |
| Instrument Name: | Agilent 6230 TOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | POSITIVE |
| MS ID: | MS002520 |
| Analysis ID: | AN002723 |
| Instrument Name: | Agilent 6230 TOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | NEGATIVE |
