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MB Sample ID: SA155071
| Local Sample ID: | s24_1 |
| Subject ID: | SU001759 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 19-87 |
| Gender: | Male and female |
| Human Race: | Caucasian |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001759 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 19-87 |
| Gender: | Male and female |
| Human Race: | Caucasian |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| s24_1 | SA155071 | FL018269 | TG | Label |
| s24_1 | SA155071 | FL018269 | b3 | Batch |
Collection:
| Collection ID: | CO001752 |
| Collection Summary: | All morning fasting urine samples were collected in sterilized plastic sealed falcons for this study and were frozen in the refrigerator at -80 °C. All the samples were defrosted at room temperature prior the analysis. The quality control (QC) sample was prepared from a mix of all urine samples that were participated in this research in equal volume, then were filled into separate containers and were stored together with other samples. A new aliquot (from individual container) of QC was used in each new day of analysis. |
| Sample Type: | Urine |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001772 |
| Treatment Summary: | Creatinine level, age, sex and clinical diagnosis were collected for study samples as meta-data. The study included urine samples from patients with bladder cancer (TG) and healthy controls (CG) matched with the TG group for age and sex. |
Sample Preparation:
| Sampleprep ID: | SP001765 |
| Sampleprep Summary: | Briefly, dilute and shoot technique was utilized. Prior to analysis, samples were defrosted at room temperature and vortexed vigorously and centrifuged at 16000 rpm for 15 min, then aliquots of the supernatant were diluted in 5-fold by deionized water with a final papaverine concentration of 0.2 µg mL-1 (IS), vortexed vigorously and centrifuged at 16000 rpm for 15 min and then aliquots of supernatant were placed into vials. |
| Processing Storage Conditions: | Room temperature |
| Extract Storage: | Room temperature |
Combined analysis:
| Analysis ID | AN002746 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu 20AD |
| Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Other |
| MS instrument name | Shimadzu LCMS-IT-TOF |
| Ion Mode | POSITIVE |
| Units | area |
Chromatography:
| Chromatography ID: | CH002028 |
| Chromatography Summary: | The apparatus consisted of a two LC-20AD pumps (Shimadzu, Japan), an automatic sample injector, coupled on-line with an LCMS-IT-TOF (Shimadzu, Japan) mass spectrometer with an electrospray ionization source. The HPLC separation was conducted on a 2.1 × 100 mm Waters Acquity UPLC BEH; 1.7 μm column (Waters, Milford MA, USA) with 2.1 × 5 mm UHPLC Guard 3PK Zorbax SB-C18 1.8 μm guard column (Agilent Technologies, USA). Two solvents were used: (A) deionized water with 0,1% formic acid, and (B) MeCN. After a 5 min hold at 5% solvent B, elution was performed according to the following conditions: from 5% B to 40% B in 20 min; to 95% B in 7 min; keeping constant for 10 min, to 5% B in 3 min, followed by 5 min of equilibration on the initial conditions. Total analysis time was 50 min. Column temperature was 35˚C. Autosampler tray temperature was 4˚C. |
| Instrument Name: | Shimadzu 20AD |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Column Pressure: | 300-500 bar |
| Column Temperature: | 35 |
| Flow Gradient: | non-linear |
| Flow Rate: | 0.3 ml/min |
| Injection Temperature: | 5 |
| Internal Standard: | papaverine |
| Sample Injection: | 5 mkl |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 50 min |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002543 |
| Analysis ID: | AN002746 |
| Instrument Name: | Shimadzu LCMS-IT-TOF |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | The apparatus consisted of a two LC-20AD pumps (Shimadzu, Japan), an automatic sample injector, coupled on-line with an LCMS-IT-TOF (Shimadzu, Japan) mass spectrometer. The column effluent was analyzed by ESI-MS. MS detection was operated in positive ionisation mode with the following parameters: detector voltage, 1.53 kV; interface voltage, +4.5 kV; CDL (curve desolvation line) temperature, 200 ˚C; block heater temperature, 200 ˚C; nebulizing gas flow (He), 1.5 L/min; drying gas pressure, 100 kPa. Full scan MS data were acquired in the range of 80–850 m/z (ion accumulation time, 30 ms; event time, 300 ms; repeat = 3. |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | +4.5 kV |
| Dry Gas Flow: | 100 kPa |
| Helium Flow: | 1.5 ml/min |
| Ion Source Temperature: | 200 ˚C |
| Ionization: | positive |
| Mass Accuracy: | 15 ppm |
| Source Temperature: | 200 ˚C |
| Cdl Temperature: | 200 ˚C |
