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MB Sample ID: SA155393
Local Sample ID: | 2285 |
Subject ID: | SU001761 |
Subject Type: | Mammal |
Subject Species: | Oryctolagus cuniculus |
Taxonomy ID: | 9986 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001761 |
Subject Type: | Mammal |
Subject Species: | Oryctolagus cuniculus |
Taxonomy ID: | 9986 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
2285 | SA155393 | FL018320 | Transgenic | Genotype |
Collection:
Collection ID: | CO001754 |
Collection Summary: | Immediately after the scarification, gastrocnemius muscle was dissected. These muscle samples were immediately frozen in liquid nitrogen, and stored at -80 degree until a metabolome analysis. |
Sample Type: | Muscle |
Treatment:
Treatment ID: | TR001774 |
Treatment Summary: | At the end of high fat diet feeding for 16 weeks (40 weeks old), after overnight fasting, rabbits were sacrificed between 08:30 and 12:30 by overdose of thiamylal sodium. |
Sample Preparation:
Sampleprep ID: | SP001767 |
Sampleprep Summary: | The samples were mixed with 1500 µL of 50% acetonitrile in water (v/v) containing internal standards (20 μM) and homogenized by a homogenizer (4500 rpm, 60 sec × 10 times). The supernatant (400 µL) was then filtrated through 5-kDa cut-off filter (ULTRAFREE-MC-PLHCC, Human Metabolome Technologies, Yamagata, Japan) to remove macromolecules. The filtrate was centrifugally concentrated and resuspended in 50 µL of ultrapure water immediately before the measurement. |
Combined analysis:
Analysis ID | AN002750 | AN002751 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | CE | CE |
Chromatography system | Agilent CE | Agilent CE |
Column | Agilent Fused silica capillary (800 mm x 50 um) | Agilent Fused silica capillary (800 mm x 50 um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Agilent CE-TOFMS | Agilent CE-TOFMS |
Ion Mode | POSITIVE | NEGATIVE |
Units | Relative Area | Relative Area |
Chromatography:
Chromatography ID: | CH002032 |
Instrument Name: | Agilent CE |
Column Name: | Agilent Fused silica capillary (800 mm x 50 um) |
Chromatography Type: | CE |
MS:
MS ID: | MS002547 |
Analysis ID: | AN002750 |
Instrument Name: | Agilent CE-TOFMS |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | - |
Ion Mode: | POSITIVE |
MS ID: | MS002548 |
Analysis ID: | AN002751 |
Instrument Name: | Agilent CE-TOFMS |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | - |
Ion Mode: | NEGATIVE |