Return to study ST001684 main page
MB Sample ID: SA155398
| Local Sample ID: | 2314 |
| Subject ID: | SU001761 |
| Subject Type: | Mammal |
| Subject Species: | Oryctolagus cuniculus |
| Taxonomy ID: | 9986 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001761 |
| Subject Type: | Mammal |
| Subject Species: | Oryctolagus cuniculus |
| Taxonomy ID: | 9986 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 2314 | SA155398 | FL018320 | Transgenic | Genotype |
Collection:
| Collection ID: | CO001754 |
| Collection Summary: | Immediately after the scarification, gastrocnemius muscle was dissected. These muscle samples were immediately frozen in liquid nitrogen, and stored at -80 degree until a metabolome analysis. |
| Sample Type: | Muscle |
Treatment:
| Treatment ID: | TR001774 |
| Treatment Summary: | At the end of high fat diet feeding for 16 weeks (40 weeks old), after overnight fasting, rabbits were sacrificed between 08:30 and 12:30 by overdose of thiamylal sodium. |
Sample Preparation:
| Sampleprep ID: | SP001767 |
| Sampleprep Summary: | The samples were mixed with 1500 µL of 50% acetonitrile in water (v/v) containing internal standards (20 μM) and homogenized by a homogenizer (4500 rpm, 60 sec × 10 times). The supernatant (400 µL) was then filtrated through 5-kDa cut-off filter (ULTRAFREE-MC-PLHCC, Human Metabolome Technologies, Yamagata, Japan) to remove macromolecules. The filtrate was centrifugally concentrated and resuspended in 50 µL of ultrapure water immediately before the measurement. |
Combined analysis:
| Analysis ID | AN002750 | AN002751 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | CE | CE |
| Chromatography system | Agilent CE | Agilent CE |
| Column | Agilent Fused silica capillary (800 mm x 50 um) | Agilent Fused silica capillary (800 mm x 50 um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent CE-TOFMS | Agilent CE-TOFMS |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Relative Area | Relative Area |
Chromatography:
| Chromatography ID: | CH002032 |
| Instrument Name: | Agilent CE |
| Column Name: | Agilent Fused silica capillary (800 mm x 50 um) |
| Chromatography Type: | CE |
MS:
| MS ID: | MS002547 |
| Analysis ID: | AN002750 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | POSITIVE |
| MS ID: | MS002548 |
| Analysis ID: | AN002751 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | NEGATIVE |
