Return to study ST001725 main page
MB Sample ID: SA162376
| Local Sample ID: | Female_Control_02 |
| Subject ID: | SU001802 |
| Subject Type: | Fish |
| Subject Species: | Danio rerio |
| Taxonomy ID: | 7955 |
| Age Or Age Range: | 1 year old |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001802 |
| Subject Type: | Fish |
| Subject Species: | Danio rerio |
| Taxonomy ID: | 7955 |
| Age Or Age Range: | 1 year old |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Female_Control_02 | SA162376 | FL019180 | Control | Sample Source |
Collection:
| Collection ID: | CO001795 |
| Collection Summary: | The right optic nerve was crushed in anesthetized, 1 yr old, wildtype fish. The fish recovered from anesthesia in fresh water and placed back into the aquarium system for 3 days. On the third day tissue was dissected from euthanized fish and “flash” frozen on dry ice in Eppendorf tubes. Samples were harvested and frozen within 10 minutes. |
| Sample Type: | Eye tissue |
Treatment:
| Treatment ID: | TR001815 |
| Treatment Summary: | No treatment |
Sample Preparation:
| Sampleprep ID: | SP001808 |
| Sampleprep Summary: | Lipid extraction was done with the Bligh and Dyer method, followed by untargeted liquid chromatography-mass spectrometry (LC MS-MS) lipid profiling using a Q-Exactive Orbitrap instrument coupled with Vanquish Horizon Binary UHPLC LC-MS system |
Combined analysis:
| Analysis ID | AN002810 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Vanquish Horizon Binary UHPLC |
| Column | Accucore Vanquish C18+ UHPLC (50 x 2.1mm,1.5um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | ug/ml |
Chromatography:
| Chromatography ID: | CH002076 |
| Instrument Name: | Vanquish Horizon Binary UHPLC |
| Column Name: | Accucore Vanquish C18+ UHPLC (50 x 2.1mm,1.5um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002605 |
| Analysis ID: | AN002810 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The lipids were identified and quantified with LipidSearch 4.2.21 and the statistical analysis was conducted through Metaboanalyst 5.0 |
| Ion Mode: | UNSPECIFIED |
