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MB Sample ID: SA164524
| Local Sample ID: | 2020_04_15_AM_HLICNeg15_HBE85_12_C |
| Subject ID: | SU001851 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001851 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Not applicable |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 2020_04_15_AM_HLICNeg15_HBE85_12_C | SA164524 | FL019603 | Control 12h | Experimental variable |
Collection:
| Collection ID: | CO001844 |
| Collection Summary: | At each time point (4, 12, 24 h), ALI basolateral media was collected and incubated 1:1 in ice-cold 100% methanol for 30 minutes on ice, vortexing every 10 minutes. Basolateral media was centrifuged at 20,000 × 𝑔 for 10 minutes at 4°C, and further diluted in 50% methanol prior to mass spectrometry plate loading. |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR001864 |
| Treatment Summary: | Highly differentiated primary human airway epithelial were infected or not with HRV-C15 for 4, 12 or 24 h. |
| Treatment Compound: | Human rhinovirus C15 |
Sample Preparation:
| Sampleprep ID: | SP001857 |
| Sampleprep Summary: | media was collected and incubated 1:1 in ice-cold 100% methanol for 30 minutes on ice, vortexing every 10 minutes. Basolateral media was centrifuged at 20,000 × 𝑔 for 10 minutes at 4°C, and further diluted in 50% methanol prior to mass spectrometry plate loading. |
Combined analysis:
| Analysis ID | AN002881 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Vanquish |
| Column | ThermoFisher Syncronis HILIC UHPLC (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Single quadrupole |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | NEGATIVE |
| Units | pmoles/l |
Chromatography:
| Chromatography ID: | CH002136 |
| Chromatography Summary: | General metabolomic run were performed on a Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ Mass Spectrometer (ThermoFisher) coupled to a Vanquish™ UHPLC System (ThermoFisher). Chromatographic separation was achieved on a Syncronis HILIC UHPLC column (2.1 mm x 100 mm x 1.7 mm, ThermoFisher) using a binary solvent system at a flow rate of 600 mL/min. Solvent A, 20 mM ammonium formate pH 3.0 in mass spectrometry grade H2O; Solvent B, mass spectrometry grade acetonitrile with 0.1% formic acid (% v/v). A sample injection volume of 2 mL was used. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | ThermoFisher Syncronis HILIC UHPLC (100 x 2.1mm,1.7um) |
| Solvent A: | 100% water; 20 mM ammonium formate, pH 3 |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS002674 |
| Analysis ID: | AN002881 |
| Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
| Instrument Type: | Single quadrupole |
| MS Type: | ESI |
| MS Comments: | The mass spectrometer was run in negative full scan mode at a resolution of 240,000 scanning from 50-750 m/z. |
| Ion Mode: | NEGATIVE |
