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MB Sample ID: SA164981
Local Sample ID: | Model mixture 13 |
Subject ID: | SU001854 |
Subject Type: | Synthetic sample |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001854 |
Subject Type: | Synthetic sample |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Model mixture 13 | SA164981 | FL019734 | AGC 1e5 - R 120K - pos | Experimental Condition |
Collection:
Collection ID: | CO001847 |
Collection Summary: | PD1074 C. elegans samples (~10 mg) were collected and lyophilized. |
Sample Type: | Worms |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR001867 |
Treatment Summary: | standard PD1047 C.elegans treatment |
Sample Preparation:
Sampleprep ID: | SP001860 |
Sampleprep Summary: | Model Mixture Sample Preparation One hundred and four selected chemical standard compounds were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used to prepare stock solutions at a concentration of 0.001 M in LC-MS grade methanol (Fisher Scientific, Pittsburgh, PA, USA). These standards were selected because they map to key metabolic pathways, some involved in cancer. If a chemical could not be completely dissolved in methanol, LC-MS grade water (Fisher Scientific, Pittsburgh, PA, USA) was added to increase solubility. A pooled sample of 104 standard compounds was diluted with methanol at a final concentration of 5 μM. PD1074 C. elegans Bio-mixture Sample Preparation Three 2.0 mm zirconium oxide beads and ~75 μL volume of 0.5 mm glass beads were added to each lyophilized PD1074 C. elegans sample (~10 mg). Samples were placed in a TissueLyser II (QIAGEN, Hilden, Germany) and homogenized at 1,800 rpm, -80 °C for 3 min. One and a half mL of 80% methanol (in water) was added to each homogenized sample. Samples were then shaken using an Isotemp high speed shaker (Fisher Scientific, Pittsburgh, PA, USA) at 1,500 rpm for 30 min, and centrifuged at 22,100 g for 5 min. The supernatant was collected, dried and stored at -80 °C. Prior to MS analysis, dried C. elegans matrices were resuspended with 1 mL LC-MS grade methanol containing the 104 standard compounds at a 5 μM concentration. |
Combined analysis:
Analysis ID | AN002884 | AN002885 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Thermo Vanquish | Thermo Vanquish |
Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Orbitrap ID-X Tribrid | Thermo Orbitrap ID-X Tribrid |
Ion Mode | POSITIVE | NEGATIVE |
Units | Da | Da |
Chromatography:
Chromatography ID: | CH002139 |
Chromatography Summary: | HILIC method |
Instrument Name: | Thermo Vanquish |
Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
Column Temperature: | 40 |
Flow Rate: | 0.4 ml/min |
Sample Injection: | 2μL |
Solvent A: | 80% water/20% acetonitrile; 0.1% formic acid; 10 mM ammonium acetate |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | HILIC |
MS:
MS ID: | MS002677 |
Analysis ID: | AN002884 |
Instrument Name: | Thermo Orbitrap ID-X Tribrid |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Samples were analyzed in positive and negative ion modes for HILIC chromatography. Spectral features were extracted from the raw data using MZmine 2.51. |
Ion Mode: | POSITIVE |
Spray Voltage: | 3.5 kV |
Automatic Gain Control: | 5e4, 1e5, 5e5 |
MS ID: | MS002678 |
Analysis ID: | AN002885 |
Instrument Name: | Thermo Orbitrap ID-X Tribrid |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Samples were analyzed in positive and negative ion modes for HILIC chromatography. Spectral features were extracted from the raw data using MZmine 2.51. |
Ion Mode: | NEGATIVE |
Spray Voltage: | -2.5 kV |
Automatic Gain Control: | 5e4, 1e5, 5e5 |