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MB Sample ID: SA174693
| Local Sample ID: | Vehicle 10 |
| Subject ID: | SU001946 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Gender: | Male |
| Animal Animal Supplier: | Charles River |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001946 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Gender: | Male |
| Animal Animal Supplier: | Charles River |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Vehicle 10 | SA174693 | FL021453 | Vehicle | Treatment |
Collection:
| Collection ID: | CO001939 |
| Collection Summary: | Right ventricular free walls were collected and snap frozen immediately after death. |
| Sample Type: | Heart |
Treatment:
| Treatment ID: | TR001958 |
| Treatment Summary: | PBS, WNK-vehicle, or WNK463 at a dose of 3 mg/kg |
Sample Preparation:
| Sampleprep ID: | SP001952 |
| Sampleprep Summary: | Samples were frozen and sent to Metabolon |
Combined analysis:
| Analysis ID | AN003031 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | relative value |
Chromatography:
| Chromatography ID: | CH002244 |
| Chromatography Summary: | Low pH polar |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002818 |
| Analysis ID: | AN003031 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Proprietary analytical software for integration and peak picking |
| Ion Mode: | POSITIVE |
