Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA174855

Local Sample ID:	Mouse liver tissue-30V
Subject ID:	SU001952
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Subject:

Subject ID:	SU001952
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
Mouse liver tissue-30V	SA174855	FL021487	Mouse liver	Source
Mouse liver tissue-30V	SA174855	FL021487	30V	MS2 CE

Collection:

Collection ID:	CO001945
Collection Summary:	Faecal samples and liver tissue were collected and immediately stored at -80 °C for further analysis.
Sample Type:	Mouse feces and liver
Storage Conditions:	-80℃

Treatment:

Treatment ID:	TR001964
Treatment Summary:	No treatment

Sample Preparation:

Sampleprep ID:	SP001958
Sampleprep Summary:	Mouse liver tissue: Six- to eight-week-old male C57BL/6 mice were fed a regular standard breeding diet and housed under standard pathogen-free (SPF) conditions. Faecal samples were collected and immediately stored at -80 °C for further analysis. After the mice were sacrificed, liver tissue was sampled, immediately frozen in liquid nitrogen, and then stored at -80 °C. The study was approved by the Animal Ethics Committee of Saiye Biology (ACU20-A027). Mouse liver tissue (20 mg) and 600 μL CH3OH/H2O (8:2, vol/vol) solvent were combined in a centrifuge tube and then homogenized (28 Hz, 60 s and 30 s) in a frozen mixed ball grinding machine (MM400, Retsch Technology, Han, Germany). The mixture was centrifuged for 10 min at 15,000 g and 4 °C, 500 μL of supernatant was transferred to a new centrifuge tube for lyophilization, and the residue was reconstituted with 100 μL of Mix-IS-MeOH.Mouse faeces: A 60 mg sample of mouse faeces and 600 μL CH3OH/H2O (8:2, vol/vol) solvent were combined in a centrifuge tube and then homogenized (28 Hz, 60 s and 30 s) in a frozen mixed ball grinding machine (MM400, Retsch Technology, Han, Germany). The mixture was centrifuged for 10 min at 15,000 g and 4 °C; then, 500 μL of supernatant was transferred to a new centrifuge tube for lyophilization, and the residue was reconstituted with 100 μL of Mix-IS-MeOH.

Sampleprep ID:

SP001958

Sampleprep Summary:

Mouse liver tissue: Six- to eight-week-old male C57BL/6 mice were fed a regular standard breeding diet and housed under standard pathogen-free (SPF) conditions. Faecal samples were collected and immediately stored at -80 °C for further analysis. After the mice were sacrificed, liver tissue was sampled, immediately frozen in liquid nitrogen, and then stored at -80 °C. The study was approved by the Animal Ethics Committee of Saiye Biology (ACU20-A027). Mouse liver tissue (20 mg) and 600 μL CH3OH/H2O (8:2, vol/vol) solvent were combined in a centrifuge tube and then homogenized (28 Hz, 60 s and 30 s) in a frozen mixed ball grinding machine (MM400, Retsch Technology, Han, Germany). The mixture was centrifuged for 10 min at 15,000 g and 4 °C, 500 μL of supernatant was transferred to a new centrifuge tube for lyophilization, and the residue was reconstituted with 100 μL of Mix-IS-MeOH.Mouse faeces: A 60 mg sample of mouse faeces and 600 μL CH3OH/H2O (8:2, vol/vol) solvent were combined in a centrifuge tube and then homogenized (28 Hz, 60 s and 30 s) in a frozen mixed ball grinding machine (MM400, Retsch Technology, Han, Germany). The mixture was centrifuged for 10 min at 15,000 g and 4 °C; then, 500 μL of supernatant was transferred to a new centrifuge tube for lyophilization, and the residue was reconstituted with 100 μL of Mix-IS-MeOH.

Combined analysis:

Analysis ID	AN003037
Analysis type	MS
Chromatography type	Reversed phase
Chromatography system	Waters Acquity
Column	Waters Acquity BEH C8 (100 x 2.1mm,1.7um)
MS Type	ESI
MS instrument type	QTOF
MS instrument name	ABI Sciex 5600+ TripleTOF
Ion Mode	POSITIVE
Units	blank-substracted abundances

Chromatography:

Chromatography ID:	CH002250
Instrument Name:	Waters Acquity
Column Name:	Waters Acquity BEH C8 (100 x 2.1mm,1.7um)
Column Temperature:	50
Flow Rate:	0.35 mL/min
Chromatography Type:	Reversed phase

MS:

MS ID:	MS002824
Analysis ID:	AN003037
Instrument Name:	ABI Sciex 5600+ TripleTOF
Instrument Type:	QTOF
MS Type:	ESI
MS Comments:	The spray voltage was 5.5 kV and the source temperature was 550 °C, Curtain gas, gas 1 and gas 2 were set at 35, 55, and 55 psi, respectively. The data was processed by XCMS, MS-DIAL, MZmine 2 and MetEx.
Ion Mode:	POSITIVE