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MB Sample ID: SA178425

Local Sample ID:MtdBlank006
Subject ID:SU002006
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Female

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Subject:

Subject ID:SU002006
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Female

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
MtdBlank006SA178425FL022207-Organ
MtdBlank006SA178425FL022207-Treatment
MtdBlank006SA178425FL022207-Group
MtdBlank006SA178425FL022207-ODMAmetabotye

Collection:

Collection ID:CO001999
Collection Summary:Urine biospecimens were collected during cross-over trial among premenopausal women conducted from 2007-2010, and details about the study design and population are published (Morimoto et al 2014; Maskarinec et al 2011; Maskarinec et al 2012). The BEAN2 trial was a cross-over study with six months on a high- and a low-soy diet, each separated by a 1-month washout period.
Sample Type:Urine

Treatment:

Treatment ID:TR002018
Treatment Summary:As part of the parent study, daidzein, equol, and ODMA concentrations were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). These previously measured concentrations were used to classify metabotypes. Each urine sample was identified as being ODMA producer/non-producer and equol producer/non-producer based on a cut-off of equol/ODMA:daidzein ratio of 0.018. All samples, except two samples, which were excluded from selection, had daidzein concentrations >2 nmol/mg creatinine, indicating sufficient presence of the precursor metabolite for metabolite detection.

Sample Preparation:

Sampleprep ID:SP002012
Sampleprep Summary:Urine biospecimens were collected during cross-over trial among premenopausal women conducted from 2007-2010, and urine sample aliquots used for this analysis were kept frozen at -80C.

Combined analysis:

Analysis ID AN003136
Analysis type MS
Chromatography type HILIC
Chromatography system Waters Acquity
Column Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
MS Type ESI
MS instrument type QTOF
MS instrument name Unspecified
Ion Mode UNSPECIFIED
Units Peak height

Chromatography:

Chromatography ID:CH002318
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
Chromatography Type:HILIC

MS:

MS ID:MS002916
Analysis ID:AN003136
Instrument Name:Unspecified
Instrument Type:QTOF
MS Type:ESI
MS Comments:See uploaded file.
Ion Mode:UNSPECIFIED
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