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MB Sample ID: SA178448
Local Sample ID: | B134u1.2 |
Subject ID: | SU002006 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Female |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002006 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Female |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
B134u1.2 | SA178448 | FL022209 | Urine | Organ |
B134u1.2 | SA178448 | FL022209 | C0 | Treatment |
B134u1.2 | SA178448 | FL022209 | C0 | Group |
B134u1.2 | SA178448 | FL022209 | Nonproducer | ODMAmetabotye |
Collection:
Collection ID: | CO001999 |
Collection Summary: | Urine biospecimens were collected during cross-over trial among premenopausal women conducted from 2007-2010, and details about the study design and population are published (Morimoto et al 2014; Maskarinec et al 2011; Maskarinec et al 2012). The BEAN2 trial was a cross-over study with six months on a high- and a low-soy diet, each separated by a 1-month washout period. |
Sample Type: | Urine |
Treatment:
Treatment ID: | TR002018 |
Treatment Summary: | As part of the parent study, daidzein, equol, and ODMA concentrations were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). These previously measured concentrations were used to classify metabotypes. Each urine sample was identified as being ODMA producer/non-producer and equol producer/non-producer based on a cut-off of equol/ODMA:daidzein ratio of 0.018. All samples, except two samples, which were excluded from selection, had daidzein concentrations >2 nmol/mg creatinine, indicating sufficient presence of the precursor metabolite for metabolite detection. |
Sample Preparation:
Sampleprep ID: | SP002012 |
Sampleprep Summary: | Urine biospecimens were collected during cross-over trial among premenopausal women conducted from 2007-2010, and urine sample aliquots used for this analysis were kept frozen at -80C. |
Combined analysis:
Analysis ID | AN003136 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Waters Acquity |
Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Unspecified |
Ion Mode | UNSPECIFIED |
Units | Peak height |
Chromatography:
Chromatography ID: | CH002318 |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
Chromatography Type: | HILIC |
MS:
MS ID: | MS002916 |
Analysis ID: | AN003136 |
Instrument Name: | Unspecified |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | See uploaded file. |
Ion Mode: | UNSPECIFIED |