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MB Sample ID: SA183609
| Local Sample ID: | MGH10 |
| Subject ID: | SU002025 |
| Subject Type: | Plant |
| Subject Species: | Zea mays |
| Taxonomy ID: | 4577 |
| Age Or Age Range: | 17 day after germination |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002025 |
| Subject Type: | Plant |
| Subject Species: | Zea mays |
| Taxonomy ID: | 4577 |
| Age Or Age Range: | 17 day after germination |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| MGH10 | SA183609 | FL022431 | Ames 27089 | Pedigree |
Collection:
| Collection ID: | CO002018 |
| Collection Summary: | Entire shoots of 17-day old maize plants were collected and immediately frozen in liquid nitrogen. All samples were collected within one hour in the middle of the day (12:00-1:00 pm). |
| Sample Type: | Plant |
| Collection Method: | Cut entire shoots from 1 cm above ground and collected into 50 ml screw cap tube and quickly thrown into liquid N2. |
| Collection Location: | University of Nebraska-Lincoln Innovation Campus greenhouse |
| Collection Frequency: | once |
| Collection Duration: | 8/16/2018 12:00 pm - 1:00 pm |
| Volumeoramount Collected: | Entire shoot of a single plant |
| Storage Conditions: | -80℃ |
| Collection Vials: | 50 ml falcon tube |
| Storage Vials: | 50 ml falcon tube |
| Collection Tube Temp: | Liquid N2 |
Treatment:
| Treatment ID: | TR002037 |
| Treatment Summary: | Seeds were surface-sterilized, imbibed, and sown on Fafard germination soil. Plants were and cultured in a greenhouse with a temperature of 18-25 °C and a 14/10 h light/dark cycle for 14 days. All inbred lines were treated equally. |
| Plant Growth Support: | Fafard germination soil |
| Plant Growth Location: | University of Nebraska-Lincoln Innovation campus greenhouse |
| Plant Plot Design: | Randomized |
| Plant Light Period: | 14/10 h light/dark cycle |
| Plant Temp: | 18-25 °C |
| Plant Watering Regime: | daily |
| Plant Nutritional Regime: | no nutrient addition during the experimental period |
| Plant Estab Date: | 2018-07-31 |
| Plant Harvest Date: | 2018-08-13 |
| Plant Growth Stage: | Juvenile |
| Plant Metab Quench Method: | Quick freezing by liquid nitrogen |
| Plant Harvest Method: | cutting |
Sample Preparation:
| Sampleprep ID: | SP002031 |
| Sampleprep Summary: | Whole samples were ground into fine powder under liquid nitrogen temperature and aliquoted 25 mg for metabolite analysis. The metabolites were extracted by methanol: water: chloroform and 50 µl of the upper aqueous phase was dried down to be derivatized by methoxyamination and trimethylsilylation. |
| Processing Storage Conditions: | Room temperature |
| Extract Storage: | Room temperature |
| Sample Resuspension: | pyridine |
| Sample Derivatization: | methoxyamination and trimethylsilylation |
| Sample Spiking: | 8.2 ug/ml ribitol |
Combined analysis:
| Analysis ID | AN003169 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890B |
| Column | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Agilent 7200 Q-TOF |
| Ion Mode | POSITIVE |
| Units | arbitrary unit (relative contents) |
Chromatography:
| Chromatography ID: | CH002342 |
| Instrument Name: | Agilent 7890B |
| Column Name: | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
| Column Temperature: | 80 |
| Flow Gradient: | None |
| Flow Rate: | 1 ml/min |
| Injection Temperature: | 230 |
| Time Program: | 80 °C 2 min, ramp up to 330°C at 15 °C/min, hold for 6 min |
| Transferline Temperature: | 280℃ |
| Sample Syringe Size: | 10 µl |
| Randomization Order: | manual |
| Chromatography Type: | GC |
MS:
| MS ID: | MS002947 |
| Analysis ID: | AN003169 |
| Instrument Name: | Agilent 7200 Q-TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| MS Comments: | MassHunter software suits |
| Ion Mode: | POSITIVE |
| Ion Source Temperature: | 250 |
| Ionization: | EI |
| Ionization Energy: | 70 eV |
