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MB Sample ID: SA184079
| Local Sample ID: | V15 |
| Subject ID: | SU002031 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 20-53 |
| Gender: | Male and female |
| Cell Strain Details: | Primary isolated human pancreatic islets |
| Cell Primary Immortalized: | primary |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002031 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 20-53 |
| Gender: | Male and female |
| Cell Strain Details: | Primary isolated human pancreatic islets |
| Cell Primary Immortalized: | primary |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| V15 | SA184079 | FL022657 | Vehicle | Group |
| V15 | SA184079 | FL022657 | Cytokines | Treatment |
Collection:
| Collection ID: | CO002024 |
| Collection Summary: | Untargeted MS with annotated ions and their signal intensities |
| Collection Protocol ID: | Methods Fu et al. FIA_MS |
| Collection Protocol Filename: | Methods Fu et al. FIA_MS |
| Sample Type: | Cultured cells |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002043 |
| Treatment Summary: | Human islets were treated with vehicle DMSO 0.5% or 5uM peptide BAD SAHBa SD for 5 hours, then treated with PBS as control and inflammatory cytokines as experimental groups. |
| Treatment Protocol Filename: | Methods Fu et al. FIA_MS |
| Treatment Compound: | BAD SAHB SD peptide, inflammatory cytokines |
| Treatment Dose: | 5 uM peptide, 10 ng/ml TNF-α, 10 ng/ml IL-1β, and 100 ng/ml IFN |
| Treatment Doseduration: | 24 hours |
| Treatment Vehicle: | DMSO and PBS |
| Cell Media: | PIMS, Prodo Labs |
Sample Preparation:
| Sampleprep ID: | SP002037 |
| Sampleprep Summary: | 150 human islets per replicate were extracted in hot 70% ethanol at 75˚C for 3 min, placed on ice, and clarified by centrifugation. Extracts from all donors were pooled and analyzed in 8 replicates by flow-injections. |
| Sampleprep Protocol Filename: | Methods Fu et al. FIA_MS |
| Extraction Method: | Islet were extracted with hot 70% ethanol at 75˚C for 3 min |
Combined analysis:
| Analysis ID | AN003178 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | Agilent 6550 Quadrupole TOF MS |
| Column | none |
| MS Type | ESI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 6550 QTOF |
| Ion Mode | NEGATIVE |
| Units | Ion signal intensity |
Chromatography:
| Chromatography ID: | CH002350 |
| Instrument Name: | Agilent 6550 Quadrupole TOF MS |
| Column Name: | none |
| Sample Injection: | 1.5 ul |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS002956 |
| Analysis ID: | AN003178 |
| Instrument Name: | Agilent 6550 QTOF |
| Instrument Type: | Single quadrupole |
| MS Type: | ESI |
| MS Comments: | Data acquisitions performed as a service of General Metabolics, processing and ion identification/ annotations performed with proprietary of General Metabolics. |
| Ion Mode: | NEGATIVE |
