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MB Sample ID: SA184111
| Local Sample ID: | SW2 |
| Subject ID: | SU002033 |
| Subject Type: | Plant |
| Subject Species: | Zea mays |
| Taxonomy ID: | 4577 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002033 |
| Subject Type: | Plant |
| Subject Species: | Zea mays |
| Taxonomy ID: | 4577 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| SW2 | SA184111 | FL022663 | Moisture treatment | Treatment |
Collection:
| Collection ID: | CO002026 |
| Collection Summary: | A total of 121 differential metabolites (75 were increased and 46 were decreased) were identified in maize leaves cultivated under conditions of stable soil water content and dry-wet cycles. |
| Sample Type: | new |
Treatment:
| Treatment ID: | TR002045 |
| Treatment Summary: | Maize plants were subjected to two irrigation treatments. The first treatment was soil moisture dry-wet cycles, which was obtained using drip irrigation (control, DW). The second treatment was negative pressure to stabilize soil water content treatment (SW), which was obtained using the negative pressure irrigation (NPI) system. |
Sample Preparation:
| Sampleprep ID: | SP002039 |
| Sampleprep Summary: | Collected samples were thawed on ice, and metabolites were extracted from 20 µL of each sample using 120 µL of precooled 50% methanol buffer. Next, the mixture of metabolites was vortexed for 1 min, followed by incubation for 10 min at room temperature, and storing at -20°C overnight. On the next day, the mixture was centrifuged at 4,000 g for 20 min and the supernatant was subsequently transferred to 96 well plates. Samples were stored at ‐80°C prior to the LC‐MS analysis. Pooled quality control (QC) samples were also prepared by combining 10 μL of each extraction mixture |
Combined analysis:
| Analysis ID | AN003180 | AN003181 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | TripleTOF 5600 | TripleTOF 5600 |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um) | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um) |
| MS Type | APCI | APCI |
| MS instrument type | Triple TOF | Triple TOF |
| MS instrument name | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | μg/100ml | μg/100ml |
Chromatography:
| Chromatography ID: | CH002352 |
| Instrument Name: | TripleTOF 5600 |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS002958 |
| Analysis ID: | AN003180 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | APCI |
| MS Comments: | XCMS software (verison 3.4.1） |
| Ion Mode: | NEGATIVE |
| MS ID: | MS002959 |
| Analysis ID: | AN003181 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | APCI |
| MS Comments: | XCMS software (verison 3.4.1） |
| Ion Mode: | POSITIVE |
