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MB Sample ID: SA184623
| Local Sample ID: | 3B |
| Subject ID: | SU002041 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002041 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 3B | SA184623 | FL022711 | Sham-operated | Group |
Collection:
| Collection ID: | CO002034 |
| Collection Summary: | Brown adipose tissue (BAT) was excised from the mice and immediately frozen in liquid nitrogen. |
| Sample Type: | Adipose tissue |
Treatment:
| Treatment ID: | TR002053 |
| Treatment Summary: | All mice were randomly allocated for surgical procedures under blinded condition. Thoracic aortic constriction (TAC) was performed in 11-week-old male mice, and sample was collected 2 weeks after surgery. Sham-operated mice underwent the same procedures, except for aortic constriction. |
Sample Preparation:
| Sampleprep ID: | SP002047 |
| Sampleprep Summary: | To extract metabolites, 40~50mg snap-frozen samples were completely homogenized by Shake Master NEO (Bio Medical Science) in 500uL of methanol containing L-methionine sulfone (Wako 502-76641), methionine sulfone (MES, Dojindo 349-01623), and D-camphor-10-sulfonic acid sodium salt (CSA, Wako 037-01032) (all at 20 μM). After adding CHCl3 (500 μl) and distilled water (200 μl) with thorough mixing, centrifugation was performed at 4,600g for 15 min at 4°C. Then the aqueous layer (300 μl) was collected and transferred to a 5-kDa cutoff filter (UltrafreeMC-PLHCC; Human Metabolome Technologies, UFC3LCCNB-HMT). Centrifugation was performed at 9,100g for 3 hours at 20°C, after which the filtrate was centrifugally concentrated and dissolved in 50μl distilled water containing reference compounds (200 μM each of 3-aminopyrrolidine and trimesate). |
Combined analysis:
| Analysis ID | AN003196 | AN003197 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | None (Direct infusion) | None (Direct infusion) |
| Chromatography system | none | none |
| Column | none | none |
| MS Type | ESI | ESI |
| MS instrument type | Single quadrupole | Single quadrupole |
| MS instrument name | Agilent 6224 TOF | Agilent 6224 TOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | nmol/g | nmol/g |
Chromatography:
| Chromatography ID: | CH002364 |
| Instrument Name: | none |
| Column Name: | none |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS002974 |
| Analysis ID: | AN003196 |
| Instrument Name: | Agilent 6224 TOF |
| Instrument Type: | Single quadrupole |
| MS Type: | ESI |
| MS Comments: | Automatic recalibration of each acquired spectrum was performed using reference masses of reference standards ([13C isotopic ion of deprotonated acetic acid dimer (2CH3COOH - H)]-, m/z 120.03841), and ([Hexakis + deprotonated acetic acid (CH3COOH - H)]-, m/z 680.03554). Exact mass data were acquired at a rate of 1.5 spectra/s over a 50-1000 m/z range. Raw CE-TOFMS data were processed using Master Hands ver. 2.17.0.8 software for the quantification of metabolites. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS002975 |
| Analysis ID: | AN003197 |
| Instrument Name: | Agilent 6224 TOF |
| Instrument Type: | Single quadrupole |
| MS Type: | ESI |
| MS Comments: | Automatic recalibration of each acquired spectrum was performed using reference masses of reference standards ([2MeOH13Cisotope]-, m/z 66.06306), and ([Hexakis(2,2-difluoroethoxy)phosphazene]-, m/z 622.028963). Exact mass data were acquired at the rate of 1.5 cycles/s over a 50 to 1,000 m/z range. Raw CE-TOFMS data were processed using Master Hands ver. 2.17.0.8 software for the quantification of metabolites. |
| Ion Mode: | POSITIVE |
