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MB Sample ID: SA188323
| Local Sample ID: | MMV030084-2 |
| Subject ID: | SU002092 |
| Subject Type: | Cultured cells |
| Subject Species: | Plasmodium falciparum |
| Taxonomy ID: | 5833 |
| Genotype Strain: | 3D7 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002092 |
| Subject Type: | Cultured cells |
| Subject Species: | Plasmodium falciparum |
| Taxonomy ID: | 5833 |
| Genotype Strain: | 3D7 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| MMV030084-2 | SA188323 | FL023184 | 10X IC50 | Concentration |
| MMV030084-2 | SA188323 | FL023184 | 2.5 hours | Treatment time |
| MMV030084-2 | SA188323 | FL023184 | MACS purified Troph | Parasite Stage |
Collection:
| Collection ID: | CO002085 |
| Collection Summary: | Cells were pelleted and the media aspirated then a 1xPBS wash that was aspirated prior to quench and extraction. |
| Sample Type: | Pf infected RBCs |
Treatment:
| Treatment ID: | TR002104 |
| Treatment Summary: | Purified iRBCs were treated with 10XIC50 drug for 2.5 hours |
Sample Preparation:
| Sampleprep ID: | SP002098 |
| Sampleprep Summary: | 90% Methanol/10% water at 50:1 solvent/cell pellet followed by vortexing and clarification via centrifugation. |
| Processing Method: | Lysis |
| Processing Storage Conditions: | 4? |
| Extraction Method: | See SA Cobbold et al JBC 2013 |
| Extract Cleanup: | Clarification via centrifugation followed by nitrogen drying |
| Extract Storage: | -80? |
| Sample Spiking: | Internal Standard 13C4, 15N1-Aspartate used for sample preparation normalizer |
Combined analysis:
| Analysis ID | AN003277 | AN003278 | AN003279 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS |
| Column | Waters XSelect HSS T3 C18 (100 x 2.1mm,2.5um) | Water XSelect HSS T3 C18 (100 x 2.1mm,2.5um) | Water XSelect HSS T3 C18 (100 x 2.1mm,2.5um) |
| MS Type | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Exactive Plus Orbitrap | Thermo Exactive Plus Orbitrap | Thermo Exactive Plus Orbitrap |
| Ion Mode | NEGATIVE | NEGATIVE | NEGATIVE |
| Units | peak area | peak area | peak area |
Chromatography:
| Chromatography ID: | CH002419 |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | Waters XSelect HSS T3 C18 (100 x 2.1mm,2.5um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002420 |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | Water XSelect HSS T3 C18 (100 x 2.1mm,2.5um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002421 |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | Water XSelect HSS T3 C18 (100 x 2.1mm,2.5um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003049 |
| Analysis ID: | AN003277 |
| Instrument Name: | Thermo Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | El-Maven for feature assignments |
| Ion Mode: | NEGATIVE |
| MS ID: | MS003050 |
| Analysis ID: | AN003278 |
| Instrument Name: | Thermo Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | El-Maven for feature assignments |
| Ion Mode: | NEGATIVE |
| MS ID: | MS003051 |
| Analysis ID: | AN003279 |
| Instrument Name: | Thermo Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | El-Maven for feature assignments |
| Ion Mode: | NEGATIVE |
