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MB Sample ID: SA189535
| Local Sample ID: | S-A128-G13-M |
| Subject ID: | SU002105 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002105 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| S-A128-G13-M | SA189535 | FL023344 | S128 | Short sample name |
| S-A128-G13-M | SA189535 | FL023344 | Thyroid | Organ |
| S-A128-G13-M | SA189535 | FL023344 | Phenytoin | Treatment |
| S-A128-G13-M | SA189535 | FL023344 | 2400 ppm | Concentration |
| S-A128-G13-M | SA189535 | FL023344 | 4 weeks | Period |
| S-A128-G13-M | SA189535 | FL023344 | 2 weeks | Recovery |
Collection:
| Collection ID: | CO002098 |
| Collection Summary: | The animal experiment was done by our cooperative partner BASF. The liver and thyroid tissue samples were delivered to us with the protection of dry ice. And all samples was stored immediately under -80℃ until further analysis. |
| Sample Type: | Tissues |
Treatment:
| Treatment ID: | TR002117 |
| Treatment Summary: | Two well-described test chemicals, i.e. Phenytoin and Propylthiouracil, were administered via the diet at each two concentrations to male Wistar rats for 2 and 4 weeks. In addition, a recovery period of 2 weeks without test substance application was included in order to monitor reversibility of the induced effects. Phenytoin was administered concentrations of 300 and 2400 ppm, Propylthiouracil was administered at concentrations of 5 and 50 ppm. Control animals received maintenance diet only without inclusion of any test substance. |
Sample Preparation:
| Sampleprep ID: | SP002111 |
| Sampleprep Summary: | Tissue metabolites were extracted according to our laboratory standard protocol. Briefly, about 100 mg liver and all received thyroid gland (2.4 - 34.1 mg) tissue materials were weighed and mixed with 5x extraction buffer (ACN / H2O: 1:1). With the help of added 4 metal balls, the tissue metabolites were separated by using a tissue slicer for 10 min at 30 Hz. After centrifugation of 2 min at 14,000 rpm, the metabolites containing supernatant were transfered to a fresh tube, and stored at -80℃ for further analysis. The targeted metabolomics was done by using MxP® Quant 500 kit (Biocrates). All analysis steps was followed by the manufactures from Biocrates. |
Combined analysis:
| Analysis ID | AN003293 |
|---|---|
| Analysis type | MS |
| Chromatography type | Unspecified |
| Chromatography system | Agilent 1290 Infinity |
| Column | MxP Quant 500 System |
| MS Type | ESI |
| MS instrument type | QTRAP |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | UNSPECIFIED |
| Units | µM |
Chromatography:
| Chromatography ID: | CH002432 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | MxP Quant 500 System |
| Chromatography Type: | Unspecified |
MS:
| MS ID: | MS003063 |
| Analysis ID: | AN003293 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | Both FIA and LC methods were used. Both positive and negative were used. The MS acquisition and Data processing were done following the manufacturer's instructions. from Biocrates |
| Ion Mode: | UNSPECIFIED |
