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MB Sample ID: SA194080
| Local Sample ID: | 200-9609_CON-IL_0.raw |
| Subject ID: | SU002145 |
| Subject Type: | Mammal |
| Subject Species: | Bos taurus |
| Taxonomy ID: | 9913 |
| Genotype Strain: | Holstein dairy cows |
| Age Or Age Range: | 4- 5 years |
| Weight Or Weight Range: | 733-797 |
| Gender: | Female |
| Animal Housing: | Tie stall |
| Animal Feed: | TMR Ad lib |
| Animal Water: | Ad libitum |
| Animal Inclusion Criteria: | lactation stage, Days in milk |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002145 |
| Subject Type: | Mammal |
| Subject Species: | Bos taurus |
| Taxonomy ID: | 9913 |
| Genotype Strain: | Holstein dairy cows |
| Age Or Age Range: | 4- 5 years |
| Weight Or Weight Range: | 733-797 |
| Gender: | Female |
| Animal Housing: | Tie stall |
| Animal Feed: | TMR Ad lib |
| Animal Water: | Ad libitum |
| Animal Inclusion Criteria: | lactation stage, Days in milk |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 200-9609_CON-IL_0.raw | SA194080 | FL023777 | - | Time |
| 200-9609_CON-IL_0.raw | SA194080 | FL023777 | CON | Treatment |
| 200-9609_CON-IL_0.raw | SA194080 | FL023777 | Lipidomics | Category |
Collection:
| Collection ID: | CO002138 |
| Collection Summary: | Blood was collected via coccygeal venipuncture after the morning milking during the baseline period and at -0.5, 0, 4, 8, 16, 24, and 48 h, relative to saline or LPS administration. Plasma samples were incubated on ice for 30 min before centrifugation. |
| Collection Protocol ID: | N/A |
| Collection Protocol Filename: | Collection_Protocol.docx |
| Collection Protocol Comments: | N/A |
| Sample Type: | Blood (plasma) |
| Collection Method: | Blood collection using venipuncture needle |
| Collection Location: | Coccygeal venipuncture |
| Collection Frequency: | At interval of -0.5, 0, 4, 8, 16, 24, and 48 h respectively |
| Collection Duration: | 1 min |
| Volumeoramount Collected: | 10ml |
| Storage Conditions: | -20℃ |
| Collection Vials: | 10 ml blood vial |
| Storage Vials: | Eppendorf tubes 2ml |
| Collection Tube Temp: | 4°C |
Treatment:
| Treatment ID: | TR002157 |
| Treatment Summary: | Sterile saline (CON; n = 5) or 2) LPS (0.375 μg of LPS/kg of BW in sterile saline; Escherichia coli O55:B5; Sigma Aldrich, St. Louis, MO; n = 5) |
| Treatment Protocol ID: | N/A |
| Treatment Protocol Filename: | N A |
Sample Preparation:
| Sampleprep ID: | SP002151 |
| Sampleprep Summary: | Untargeted lipidomics samples were prepared by dissolving in dicholormethane/ methanol ( MeOH; 2:1). Untargeted metabolomics was done by centrifugation, samples were re-suspended with 60% ACN prior to analysis. FOR details please refer to publication Wang et al 2022. |
Combined analysis:
| Analysis ID | AN003362 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Vanquish UHPLC system |
| Column | Accucore C30 (150 x 2.1mm,2.6um) |
| MS Type | ESI |
| MS instrument type | Single quadrupole |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | Normalized ion intensity |
Chromatography:
| Chromatography ID: | CH002488 |
| Chromatography Summary: | Chromatographic separation was performed on a Vanquish UHPLC system with an Accucore C30, 2.6 μm column (2.1 mm id × 150mm) coupled to a Q Exactive™ Hybrid Quadrupole-Orbitrap High Resolution Mass Spectrometer (Thermo Fisher Scientific, San Jose, CA) and generated data was processed using LipidSearch™ software version 4.1 (Thermo Scientific), |
| Methods Filename: | Genenral_method_for_Untargeted_Metabolomics_Mar2020.docx |
| Instrument Name: | Vanquish UHPLC system |
| Column Name: | Accucore C30 (150 x 2.1mm,2.6um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003131 |
| Analysis ID: | AN003362 |
| Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
| Instrument Type: | Single quadrupole |
| MS Type: | ESI |
| MS Comments: | Please refer to publication Wang et al 2022. |
| Ion Mode: | UNSPECIFIED |
