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MB Sample ID: SA194679
| Local Sample ID: | S125dCtrl_10 |
| Subject ID: | SU002150 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002150 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| S125dCtrl_10 | SA194679 | FL023837 | WT | Genotype |
| S125dCtrl_10 | SA194679 | FL023837 | M | Sex |
| S125dCtrl_10 | SA194679 | FL023837 | 125 | Age (d) |
Collection:
| Collection ID: | CO002143 |
| Collection Summary: | Murine heart tissue was excised and snap frozen in liquid N2. |
| Sample Type: | Heart |
Treatment:
| Treatment ID: | TR002162 |
| Treatment Summary: | N/A |
Sample Preparation:
| Sampleprep ID: | SP002156 |
| Sampleprep Summary: | 15 mg of cardiac tissue was homogenized in 80% methanol (Sigma) using Tissue Tearer (BioSpec) on dry ice. Samples were incubated at -80ºC for 4 hours. Homogenates were then centrifuged at 14,000 rfc for 20 min at 4ºC. The supernatant was extracted and stored at -80ºC for mass spectroscopy (Weill Cornell Medicine Meyer Cancer Center Proteomics & Metabolomics Core Facility). Peak intensities for metabolites were screened for missing values and analyzed by using MetaboAnalyst software (version 5.0). |
Combined analysis:
| Analysis ID | AN003370 | AN003371 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak Intensity | Peak Intensity |
Chromatography:
| Chromatography ID: | CH002492 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS003137 |
| Analysis ID: | AN003370 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | POSITIVE |
| MS ID: | MS003138 |
| Analysis ID: | AN003371 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | NEGATIVE |
