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MB Sample ID: SA194685
Local Sample ID: | S75dCtrl_4 |
Subject ID: | SU002150 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002150 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
S75dCtrl_4 | SA194685 | FL023839 | WT | Genotype |
S75dCtrl_4 | SA194685 | FL023839 | M | Sex |
S75dCtrl_4 | SA194685 | FL023839 | 75 | Age (d) |
Collection:
Collection ID: | CO002143 |
Collection Summary: | Murine heart tissue was excised and snap frozen in liquid N2. |
Sample Type: | Heart |
Treatment:
Treatment ID: | TR002162 |
Treatment Summary: | N/A |
Sample Preparation:
Sampleprep ID: | SP002156 |
Sampleprep Summary: | 15 mg of cardiac tissue was homogenized in 80% methanol (Sigma) using Tissue Tearer (BioSpec) on dry ice. Samples were incubated at -80ºC for 4 hours. Homogenates were then centrifuged at 14,000 rfc for 20 min at 4ºC. The supernatant was extracted and stored at -80ºC for mass spectroscopy (Weill Cornell Medicine Meyer Cancer Center Proteomics & Metabolomics Core Facility). Peak intensities for metabolites were screened for missing values and analyzed by using MetaboAnalyst software (version 5.0). |
Combined analysis:
Analysis ID | AN003370 | AN003371 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Thermo Vanquish | Thermo Vanquish |
Column | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak Intensity | Peak Intensity |
Chromatography:
Chromatography ID: | CH002492 |
Instrument Name: | Thermo Vanquish |
Column Name: | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
Chromatography Type: | HILIC |
MS:
MS ID: | MS003137 |
Analysis ID: | AN003370 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | - |
Ion Mode: | POSITIVE |
MS ID: | MS003138 |
Analysis ID: | AN003371 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | - |
Ion Mode: | NEGATIVE |