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MB Sample ID: SA202449
| Local Sample ID: | ChR_1WPC_PS_POS_(3) |
| Subject ID: | SU002196 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002196 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| ChR_1WPC_PS_POS_(3) | SA202449 | FL024618 | ChR 1WPC PS | Factor |
Collection:
| Collection ID: | CO002189 |
| Collection Summary: | Three-month-old Thy1-Chr2-eYFP and C57BL6/J mice were anesthetized by intraperitoneal injection of Ketamine/Xylazine cocktail. Before crush, eyes received topical anesthesia using 0.5/% proparacaine. A 1 mm hole in the superior-temporal conjunctiva was made, the muscle tissue was separated, and the optic nerve was exposed. The optic nerve was then crushed using Dumont #5 forceps (Fine Science Tools, Foster City, CA, USA) at approximately 0.5-1 mm behind the globe without damaging retinal vessels or affecting the blood supply. Mice of either sex were randomly assigned into either the optogenetic stimulation group or the control group. The mice in the optogenetic stimulation group received blue light stimulation (~470 nm) at 1 HZ frequency while the mice in the control group were kept in a normal 12h light/dark environment. The mice were kept in the stimulated environment one or two weeks after crush according to the experimental design. |
| Sample Type: | Eye tissue |
Treatment:
| Treatment ID: | TR002208 |
| Treatment Summary: | No treatment |
Sample Preparation:
| Sampleprep ID: | SP002202 |
| Sampleprep Summary: | Optic nerves from three mice were pooled per condition prior to metabolite extraction. Untargeted metabolomics was performed and analyzed using high-performance liquid chromatography and mass spectrometry using a Q-Exactive Orbitrap instrument coupled with a Vanquish Horizon Binary UHPLC LC-MS system. |
Combined analysis:
| Analysis ID | AN003454 | AN003455 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Thermo Accucore C18+ (100 x 2.1mm,1.5um) | Thermo Accucore C18+ (100 x 2.1mm,1.5um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | μg/mL | μg/mL |
Chromatography:
| Chromatography ID: | CH002552 |
| Chromatography Summary: | Positive Ion mode |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Thermo Accucore C18+ (100 x 2.1mm,1.5um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002553 |
| Chromatography Summary: | Negative Ion mode |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Thermo Accucore C18+ (100 x 2.1mm,1.5um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003217 |
| Analysis ID: | AN003454 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Metabolites were identified using Compound Discoverer 3.3 software. Protein quantification was performed and resulting peak intensities were used to quantify metabolite levels. Corrected peak intensities were imported to MetaboAnalyst 5.0 for statistical analysis. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003218 |
| Analysis ID: | AN003455 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Metabolites were identified using Compound Discoverer 3.3 software. Protein quantification was performed and resulting peak intensities were used to quantify metabolite levels. Corrected peak intensities were imported to MetaboAnalyst 5.0 for statistical analysis. |
| Ion Mode: | NEGATIVE |
