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MB Sample ID: SA203011
Local Sample ID: | Cell-Oxa-48h-1 |
Subject ID: | SU002201 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Strain Details: | A2780 ovarian cancer cells |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002201 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Strain Details: | A2780 ovarian cancer cells |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Cell-Oxa-48h-1 | SA203011 | FL024986 | lysate | sample_type |
Cell-Oxa-48h-1 | SA203011 | FL024986 | oxaliplatin | treatment_type |
Cell-Oxa-48h-1 | SA203011 | FL024986 | 48 | treatment_time |
Cell-Oxa-48h-1 | SA203011 | FL024986 | A2780 | cell_line |
Collection:
Collection ID: | CO002194 |
Collection Summary: | A2780 cells were purchase from Sigma-Aldrich and derived from the European Collection of Authenticated Cell Culture (human ovarian carcinoma; catalogue no.: 93112519; lot no.13J012, passage no.: P + 9). A2780cp cells were kindly provided by Dr Simona Bracini and were obtained via prolonged exposition to sub-lethal dose of cisplatin. Cells were maintained in RPMI1640 medium supplemented with 2 mM glutamine, 10% of FCS and antibiotics at 37 °C in a 5% CO2 atmosphere and sub-cultured twice weekly. Split 1:5 (3–6 × 10^4 cells per mL). |
Sample Type: | Cultured cells |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR002213 |
Treatment Summary: | For drug treatment, 2.7 *10^6 A2780 cells were seeded in p100 plate and after 24 hours were exposed to a concentration of the three Pt-drugs, i.e. CIS, OXA and CARBO equal to their 72 h-exposure IC50 value (2.5, 55 and 0.46 uM, respectively). The drug incubations were stopped at two different time points, 24 h and 48 h. The A2780cp cells were exposed to a concentration of cisplatin equal to their 72 h-exposure IC50 value (15 uM). The drug incubation was stopped after 48 h of treatment. |
Treatment: | Cisplatin, carboplatin and oxaliplatin |
Cell Media: | RPMI1640 |
Sample Preparation:
Sampleprep ID: | SP002207 |
Sampleprep Summary: | Frozen samples were thawed at room temperature and shaken before use. For cell lysates, 50 μl of 2H2O were added to 450 μl of each lysate sample. In the case of cell culture media, 300 μl of sodium phosphate buffer (70 mM Na2HPO4; 20% v/v 2H2O; 4.6 mM TMSP, pH 7.4) was added to 300 μl of each medium sample. The mixtures were homogenized by vortexing for 30 s and transferred into 5 mm NMR tubes (Bruker BioSpin srl) for analysis. |
Analysis:
MB Sample ID: | SA203011 |
Analysis ID: | AN003464 |
Laboratory Name: | Metabolomics |
Analysis Type: | NMR |
Results File: | ST002116_AN003464_Results.txt |
Units: | ppm |
NMR:
NMR ID: | NM000235 |
Analysis ID: | AN003464 |
Instrument Name: | Bruker Advance 600 MHZ |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-1H |
Spectrometer Frequency: | 600 MHz |
NMR Tube Size: | 5,00 mm |
Water Suppression: | water presaturation |
Temperature: | 300 K for lysates and 31o for media |