Return to study ST002149 main page
MB Sample ID: SA205912
| Local Sample ID: | Cdiff6 |
| Subject ID: | SU002235 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Clostridioides difficile ATCC43255 |
| Age Or Age Range: | 6 weeks |
| Gender: | Male and female |
| Animal Animal Supplier: | Massachusetts Host-Microbiome Center Gnotobiotic Resource |
| Animal Housing: | Germfree |
| Animal Water: | sterile, ad libitum |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002235 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Clostridioides difficile ATCC43255 |
| Age Or Age Range: | 6 weeks |
| Gender: | Male and female |
| Animal Animal Supplier: | Massachusetts Host-Microbiome Center Gnotobiotic Resource |
| Animal Housing: | Germfree |
| Animal Water: | sterile, ad libitum |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Cdiff6 | SA205912 | FL025268 | C. difficile monocolonized | Treatment |
Collection:
| Collection ID: | CO002228 |
| Collection Summary: | Cecal contents |
| Sample Type: | Cecum |
| Collection Method: | Snap freeze in cryovials on liquid nitrogen |
| Storage Conditions: | -80℃ |
| Additives: | None |
Treatment:
| Treatment ID: | TR002247 |
| Treatment Summary: | Gnotobiotic mice (baseline controls) or mono- or co-colonized with C. difficile, and the commensal P. bifermentans or C. sardiniense. |
| Treatment Dose: | gavage with 1000 spores of ATCC43255 for C. difficile infection; 200uL of overnight culture for commensal species |
Sample Preparation:
| Sampleprep ID: | SP002241 |
| Sampleprep Summary: | Cecum was dissected from mice and contents immediately transferred into cryovials for snap freezing on liquid nitrogen. Materials stored at -80*C until shipment to Metabolon, on dry ice, for analyses. |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN003519 |
|---|---|
| Analysis type | MS |
| Chromatography type | Unspecified |
| Chromatography system | Unspecified |
| Column | Unspecified |
| MS Type | Unspecified |
| MS instrument type | Unspecified |
| MS instrument name | Metabolon |
| Ion Mode | UNSPECIFIED |
| Units | OriginalScale MS Units |
Chromatography:
| Chromatography ID: | CH002598 |
| Chromatography Summary: | None |
| Instrument Name: | Unspecified |
| Column Name: | Unspecified |
| Chromatography Type: | Unspecified |
MS:
| MS ID: | MS003277 |
| Analysis ID: | AN003519 |
| Instrument Name: | Metabolon |
| Instrument Type: | Unspecified |
| MS Type: | Unspecified |
| MS Comments: | Metabolon global untargeted screen of cecal contents |
| Ion Mode: | UNSPECIFIED |
