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MB Sample ID: SA210456
Local Sample ID: | 14_WT_10w_pos |
Subject ID: | SU002281 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002281 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
14_WT_10w_pos | SA210456 | FL025709 | WT | Genotype |
14_WT_10w_pos | SA210456 | FL025709 | 10w_WT | Batch |
Collection:
Collection ID: | CO002274 |
Collection Summary: | Mice were euthanized. Then, ears were collected and stored at -80˚C after rapid freezing in liquid nitrogen. |
Sample Type: | skin |
Treatment:
Treatment ID: | TR002293 |
Treatment Summary: | Mice were bred and maintained in the Keio specific pathogen-free facility, with a 12 h light/12 h dark cycle and food (CLEA Rodent Diet CE-2, CLEA Japan, Tokyo, Japan) and water available ad libitum. |
Sample Preparation:
Sampleprep ID: | SP002287 |
Sampleprep Summary: | Frozen ear was pulverized by a metal cone using MULTI-BEADS SHOCKER MB1200 (YASUI KIKAI, Osaka, Japan), and dissolved in 1 ml methanol. The resulting solvents were transferred to a 2 ml glass tube, and 500 µl CHCl3 in internal standard (1 µM d9-EOS d18:1/32:0/18:2 (Cayman, Ann Arbor, MI, USA)) was added followed by incubation at -30˚C for 16 h. After centrifugation (2,000 g, 4˚C, 10 min), A total of 200 µl supernatants were dissolved in 100 µl methanol, and 20 μl MilliQ was added. After 15 min of incubation at room temperature, the tubes were centrifuged (2,000 g, 20˚C, 10 min). The supernatants were subjected to untargeted LC-MS/MS analysis. |
Combined analysis:
Analysis ID | AN003593 | AN003594 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Waters Acquity UPLC | Waters Acquity UPLC |
Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | Triple TOF | Triple TOF |
MS instrument name | ABI Sciex 6600 TripleTOF | ABI Sciex 6600 TripleTOF |
Ion Mode | NEGATIVE | POSITIVE |
Units | CPM/mg | CPM/mg |
Chromatography:
Chromatography ID: | CH002655 |
Chromatography Summary: | LC gradient consisted of holding solvent (A/B: 100/0) for 1 min, then linearly converting to solvent (A/B: 60/40) for 4 min, linearly converting to solvent (A/B:36/64) for 2.5 min and holding for 4.5 min, then linearly converting to solvent (A/B: 17.5/82.5) for 0.5 min, linearly converting to solvent (A/B: 15/85) for 8.5 min, and linearly converting to solvent (A/B: 5/95) for 1 min followed by returning to solvent (A/B: 100/0) and holding for 5 min for re-equilibration. |
Instrument Name: | Waters Acquity UPLC |
Column Name: | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
Column Temperature: | 45 |
Flow Gradient: | LC gradient consisted of holding solvent (A/B: 100/0) for 1 min, then linearly converting to solvent (A/B: 60/40) for 4 min, linearly converting to solvent (A/B:36/64) for 2.5 min and holding for 4.5 min, then linearly converting to solvent (A/B: 17.5/82.5) for 0.5 min, linearly converting to solvent (A/B: 15/85) for 8.5 min, and linearly converting to solvent (A/B: 5/95) for 1 min followed by returning to solvent (A/B: 100/0) and holding for 5 min for re-equilibration. |
Flow Rate: | 0.30mL/min |
Solvent A: | 20% methanol/20% acetonitrile/60% water; 5 mM ammonium acetate; 10 nM EDTA |
Solvent B: | 100% isopropanol; 5 mM ammonium acetate; 10 nM EDTA |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS003348 |
Analysis ID: | AN003593 |
Instrument Name: | ABI Sciex 6600 TripleTOF |
Instrument Type: | Triple TOF |
MS Type: | ESI |
MS Comments: | MS-DIAL was used for peak picking and alignment |
Ion Mode: | NEGATIVE |
MS ID: | MS003349 |
Analysis ID: | AN003594 |
Instrument Name: | ABI Sciex 6600 TripleTOF |
Instrument Type: | Triple TOF |
MS Type: | ESI |
MS Comments: | MS-DIAL was used for peak picking and alignment |
Ion Mode: | POSITIVE |