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MB Sample ID: SA227173
Local Sample ID: | XW37N_liver |
Subject ID: | SU002401 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002401 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
XW37N_liver | SA227173 | FL027585 | Cox10_KO | Genotype |
Collection:
Collection ID: | CO002394 |
Collection Summary: | Liver was frozen on liqN2. Liver was crushed on liqN2, and metabolites extracted with 80% meOH. Plasma was extracted by adding 10X cold acetone. MeOH was dried, and samples derivatized to form methoxime-TBDMS adducts and immediately injected onto the GCMS. |
Sample Type: | Liver |
Treatment:
Treatment ID: | TR002413 |
Treatment Summary: | Mice were implanted with a jugular vein catheter. Conscious mice were infused with [U-13C] glucose for 4 hours. |
Sample Preparation:
Sampleprep ID: | SP002407 |
Sampleprep Summary: | 80% methanol containing metabolites was dried overnight in a speedvac. Dried pellets were derivatized using methoxyamine and MTBSTFA. Samples were immediately injected. |
Combined analysis:
Analysis ID | AN003782 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 7890B |
Column | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
MS Type | EI |
MS instrument type | Single quadrupole |
MS instrument name | Agilent 5977A |
Ion Mode | POSITIVE |
Units | peak area |
Chromatography:
Chromatography ID: | CH002796 |
Chromatography Summary: | 60 °C for 1 min then 10 °C/min to 320 °C for 1 min Run Time 28 min 9 min (Post Run) 320 °C |
Methods Filename: | acqmeth_(1).txt |
Instrument Name: | Agilent 7890B |
Column Name: | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
Flow Rate: | 104.48 mL/min |
Injection Temperature: | 60 |
Internal Standard: | Norvaline |
Sample Injection: | Splitless |
Chromatography Type: | GC |
MS:
MS ID: | MS003525 |
Analysis ID: | AN003782 |
Instrument Name: | Agilent 5977A |
Instrument Type: | Single quadrupole |
MS Type: | EI |
MS Comments: | MS scan from 75-1000 m/z Data processed using Agilent Enhanced Data Analysis Peaks were autointegrated and confirmed by hand. |
Ion Mode: | POSITIVE |