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MB Sample ID: SA227182
| Local Sample ID: | XW73R_plasma_4h |
| Subject ID: | SU002401 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002401 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| XW73R_plasma_4h | SA227182 | FL027586 | Cox10_WT | Genotype |
Collection:
| Collection ID: | CO002394 |
| Collection Summary: | Liver was frozen on liqN2. Liver was crushed on liqN2, and metabolites extracted with 80% meOH. Plasma was extracted by adding 10X cold acetone. MeOH was dried, and samples derivatized to form methoxime-TBDMS adducts and immediately injected onto the GCMS. |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR002413 |
| Treatment Summary: | Mice were implanted with a jugular vein catheter. Conscious mice were infused with [U-13C] glucose for 4 hours. |
Sample Preparation:
| Sampleprep ID: | SP002407 |
| Sampleprep Summary: | 80% methanol containing metabolites was dried overnight in a speedvac. Dried pellets were derivatized using methoxyamine and MTBSTFA. Samples were immediately injected. |
Combined analysis:
| Analysis ID | AN003782 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890B |
| Column | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5977A |
| Ion Mode | POSITIVE |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH002796 |
| Chromatography Summary: | 60 °C for 1 min then 10 °C/min to 320 °C for 1 min Run Time 28 min 9 min (Post Run) 320 °C |
| Methods Filename: | acqmeth_(1).txt |
| Instrument Name: | Agilent 7890B |
| Column Name: | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
| Flow Rate: | 104.48 mL/min |
| Injection Temperature: | 60 |
| Internal Standard: | Norvaline |
| Sample Injection: | Splitless |
| Chromatography Type: | GC |
MS:
| MS ID: | MS003525 |
| Analysis ID: | AN003782 |
| Instrument Name: | Agilent 5977A |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | MS scan from 75-1000 m/z Data processed using Agilent Enhanced Data Analysis Peaks were autointegrated and confirmed by hand. |
| Ion Mode: | POSITIVE |
